A kind of bilberry tissue culture proliferation medium and preparation method thereof
A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry
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Embodiment 1
[0029] The improved WPM medium includes the following components:
[0030] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;
[0031] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;
[0032] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;
[0033] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.
[0034] The above -mentioned proliferation and cultivation system preparation methods are as follows:
[0035] (1) Preparation of improved WPM medium:
[0036] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrat...
Embodiment 2
[0049] The improved WPM medium includes the following components:
[0050] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;
[0051] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;
[0052] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;
[0053] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.
[0054] The method of proliferation and cultivation qualifications is as follows:
[0055] (1) Preparation of improved WPM medium:
[0056] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrate, 5.95g ammonium su...
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