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The method of biocatalytic method to increase the content of the active ingredient in the Radix Radix Radix Radix Isatidis extract

A technology of biocatalysis and active ingredients, which is applied in the field of biocatalysis to increase the content of active ingredients in Radix isatidis extract, and can solve problems such as affecting the therapeutic effect of Radix isatidis preparations and the content of antiviral ingredients in Radix isatidis is not ideal. , to achieve the effect of increased paste yield, easy operation, and simple method and process

Active Publication Date: 2016-01-13
HEBEI JINNIU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Radix isatidis preparations are mostly made of Radix isatidis extract, and Radix isatidis extract is generally obtained by extracting Radix isatidis slices with water and ethanol precipitation. The extract of Radix isatidis obtained by this method does not contain enough antiviral ingredients. Ideal, thus affecting the therapeutic effect of Radix isatidis preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100 kg of Banlangen decoction pieces, feed them into the extraction tank, add 800 L of water to infiltrate for 1 hour, heat to 45 ° C, adjust the pH value to 4.5 with sodium carbonate and acetic acid, add cellulase (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., Food grade, 100000u / ml) 3×10 6 U, add protease (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., food grade, 50000u / ml) 8×10 5 U, constant temperature, stirring, enzymatic hydrolysis for 1 hour, continue to heat to 100°C and decoct (inactivate) for 1 hour, filter, and extract the concoction; add 600L of water, decoct at 100°C for 1 hour, filter, and extract the concoction. Combine the two concoctions into a double-effect concentration tank, adjust the temperature to 70°C, adjust the pH value to 6.5 with sodium carbonate and acetic acid, add medium-temperature α-amylase (purchased from Anqiu Enzyme Factory in Shandong Province, food grade, 2000u / ml) 8×10 5 U, liquefied for ...

Embodiment 2

[0024] Take 100 kg of Banlangen decoction pieces, feed them into the extraction tank, add 800 L of water to infiltrate for 1 hour, heat to 40 ° C, adjust the pH value to 6 with sodium carbonate and acetic acid, add cellulase (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., Food grade, 100000u / ml) 1×10 6 U, add protease (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., food grade, 50000u / ml) 10×10 5 U, constant temperature, stirring, enzymatic hydrolysis for 1 hour, continue to heat to 100°C and decoct (inactivate) for 1 hour, filter, and extract the concoction; add 600L of water, decoct at 100°C for 1 hour, filter, and extract the concoction. Combine the two medicine juices into the double-effect concentration tank, adjust the temperature to 60°C, adjust the pH value to 6 with sodium carbonate and acetic acid, add medium-temperature α-amylase (purchased from Anqiu Enzyme Preparation Factory, Shandong Province, food grade, 2000u / ml) 7×10 5 U, l...

Embodiment 3

[0026] Take 100 kg of Banlangen decoction pieces, feed them into the extraction tank, add 800 L of water to infiltrate for 1 hour, heat to 30 ° C, adjust the pH value to 3.5 with sodium carbonate and acetic acid, add cellulase (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., Food grade, 100000u / ml) 5×10 6 U, add protease (purchased from Shandong Taian Xindeli Bioengineering Co., Ltd., food grade, 50000u / ml) 5×10 5 U, constant temperature, stirring, enzymatic hydrolysis for 1 hour, continue to heat to 100°C and decoct (inactivate) for 1 hour, filter, and extract the concoction; add 600L of water, decoct at 100°C for 1 hour, filter, and extract the concoction. Combine the two concoctions into a double-effect concentration tank, adjust the temperature to 65°C, adjust the pH value to 7 with sodium carbonate and acetic acid, add medium-temperature α-amylase (purchased from Anqiu Enzyme Preparation Factory, Shandong Province, food grade, 2000u / ml) 6×10 5 U, liquef...

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Abstract

The invention discloses a method for improving the content of an effective ingredient epigoitrin in a radix isatidis extract via a biological catalysis method. Compared with the traditional water extraction and alcohol precipitation method, according to the method disclosed by the invention, the content of the effective ingredient epigoitrin in the radix isatidis extract at least doubled by adopting a three-enzyme method, i.e., cellulase, protease and amylase; and in addition, the extract yield is substantially improved.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine extraction, in particular to a biocatalytic method for increasing the content of the active ingredient, yichun, in Radix isatidis extract. technical background [0002] As we all know, Radix Isatidis is an ideal traditional Chinese medicine for treating viral colds and mumps. Radix Radix is ​​cold in nature and bitter in taste. In the past, indigo, indirubin and other ingredients were considered to be effective antiviral ingredients, but current research shows that indigo and indirubin have no antiviral activity, and the effective parts of antiviral effect are total alkaloids, among which Yichun is Sulfur-containing alkaloids produced in Radix isatidis, which is one of the main components of Radix isatidis anti-influenza. [See literature: 1. Research progress on antiviral active ingredients of Radix isatidis—Chinese Medicine Information, Volume 22, Issue 5, 2005, pages37-40; 3. Pharma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/195A61K36/315A61P31/16A61P31/14
Inventor 郝秋实郝炳水
Owner HEBEI JINNIU PHARMA
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