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Sugarcane anthocyanin adjustment gene ScRS and application thereof

A technology for regulating genes and anthocyanins, applied in application, genetic engineering, plant genetic improvement, etc., to achieve high food safety, elimination of ecological risks, and good color rendering effects

Inactive Publication Date: 2014-08-06
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently widely used marker genes such as GUS and GFP have certain deficiencies and defects, for example, they have no practical application value except for reporting function; The protein needs to consume plant assimilates, but it is not beneficial to plant growth, etc., and the anthocyanin transcription factor gene can not only play the function of marker gene, but also plays an important role in the growth of plants. Therefore, the expression of anthocyanin related genes Research is particularly important

Method used

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  • Sugarcane anthocyanin adjustment gene ScRS and application thereof
  • Sugarcane anthocyanin adjustment gene ScRS and application thereof
  • Sugarcane anthocyanin adjustment gene ScRS and application thereof

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Embodiment 1

[0027] A sugarcane anthocyanin regulatory gene SCRS and applications thereof, specifically including sugarcane anthocyanin-regulated genes SCRS cloning and genetic transformation, as follows:

[0028] (1) Design of PCR primers: Query maize on NCBI RS (GenBank: X15806.1), design primers at the start codon and stop codon positions according to the sequence, the sequence is:

[0029] RSF1: 5'-ATGGCCGTTTCACGCTTCC-3'; RSR1: 5'-TCACCGCTTCCCTATAGC-3'.

[0030] (2) Extraction of total RNA from sugarcane leaves

[0031] 1. Get fresh sugarcane leaves, wipe the surface of the leaves with 75% alcohol, and grind them into powder quickly under the action of liquid nitrogen. Transfer the ground material to a 1.5 mL Eppendorf centrifuge tube with 1 mL TRIZOL reagent added in advance, vibrate and mix vigorously, place at room temperature for 5 min, and centrifuge at 12 000 rpm for 5 min at 4 °C.

[0032] ②Carefully draw 800 μl of the supernatant, transfer it into a new 1.5 mL Eppendorf...

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Abstract

The invention provides a sugarcane anthocyanin adjustment gene ScRS and application thereof, belonging to the field of biotechnology. The adjustment gene ScRS has a sequence as shown in SEQ ID NO.1, a primer is designed according to a gene sequence of a corn anthocyanin transcription factor RS, and a full-length sequence of an anthocyanin transcription factor ScRS is obtained from sugarcane by adopting a homological cloning technology; the length of an opening reading frame of the sugarcane gene ScRS is 1722bp, and 573 amino acids are coded; the E value is equal to zero in BLAST homological search of a gene related with numerous corn anthocyanins, and the situation shows that a cloned gene is really the gene related with anthocyanins; enzyme cutting sites Kpn I and Sal I are added to two ends of the ScRS sequence respectively to construct a plant expression vector pCAMBIA1301-35SN-ScRS, sugarcane callus tissues are bombarded and transformed through a gene gun, the transformed callus tissues become bright purple red, and the situation further proves that the cloned gene has a function of developing the color of the sugarcane callus tissues.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a sugarcane anthocyanin regulation gene SCRS and its application. Background technique [0002] Anthocyanins, also known as anthocyanins and anthocyanins, are a class of plant-derived water-soluble pigments that are ubiquitous in nature. Anthocyanins are also the main factor for the coloration of plants, and the different pH values ​​in the plant vacuoles are the key to affecting the different colors of plant flowers. Anthocyanins in nature can not only make plants present rich and colorful colors, which is beneficial to plant pollination, but also protect plant fruits from ultraviolet rays. Previous studies have shown that anthocyanins have strong antioxidant capacity, which is 20 times higher than vitamin C and 50 times higher than vitamin E. It is an effective scavenger of free radicals, and has high nutritional and health functions in the prevention of cardiovascular diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 陈平华施桂姣高三基张卓陈忠伟王恒波项慰刘迪邰连赛林冰陈利平许莉萍陈如凯
Owner FUJIAN AGRI & FORESTRY UNIV
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