Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cultivation method for observing Plasmodium infestation of Brassicaceae plants

A technology of Brassicaceae and Plasmodium, applied in the field of plant pathology research, can solve the problems of unclear observation results, many interference factors, complicated operations, etc., and achieve the effect of convenient and clear observation, high transparency of root hairs, and convenient observation

Inactive Publication Date: 2016-07-06
云南省农业科学院园艺作物研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better control over crops by growing these roots inside special solutions that help protect it from harmful bacteria or paraphernalides during its growth cycle. By pulling up affected plant parts like leaves, stems, tubers, etc., we see how this new method works effectively without damaging their roots. These experiments provide valuable insights into the disease causing mechanism behind Pachyrosporum spp.

Problems solved by technology

This patents discuss how different methods for studying the growth conditions affecting the formation of plastid organisms during the period from germinal phase until reproduction occurs. However, current experimental techniques have limitations with regards their effectiveness towards controlling these processes. There exists an unmet challenge related to understanding the mechanism behind the development of new ways to prevent damage due to Pisma cocrophy causing severe economic losses worldwide through its transmission and propagating within crops like vegetables.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cultivation method for observing Plasmodium infestation of Brassicaceae plants
  • Cultivation method for observing Plasmodium infestation of Brassicaceae plants
  • Cultivation method for observing Plasmodium infestation of Brassicaceae plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take fresh root tissue of Cruciferous plants infected by Plasmodium brassicae and crush it, filter it through gauze with a pore size of 25 μm, and centrifuge the filtrate at a speed of 3000 r / min for 9 minutes to remove Supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant solution, and then distilled water was added to the precipitate to suspend the precipitate until 1×10 7 CFU / mL of Plasmodium brassicae bacteria liquid, stored at 4°C for later use;

[0024] (2) Sow sterilized cruciferous seeds in the sterilized quartz sand, soak the quartz sand and seeds with MS basic culture solution, and put them under the light intensity of 100μmolphotons / (m 2 .s), light time of 16h, temperature of 22°C, and humidity of 75% or more, cultivate until ...

Embodiment 2

[0031] (1) Take fresh root tissue of Cruciferous plants infected by Plasmodium brassicae and crush it, filter it through gauze with a pore size of 25 μm, and centrifuge the filtrate at a speed of 3000 r / min for 9 minutes to remove Supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant solution, and then distilled water was added to the precipitate to suspend the precipitate until 1×10 7 CFU / mL of Plasmodium brassicae bacteria liquid, stored at 4°C for later use;

[0032] (2) Sow sterilized cruciferous seeds in the sterilized quartz sand, soak the quartz sand and seeds with MS basic culture solution, and put them under the light intensity of 100μmolphotons / (m 2 .s), the light time is 18h, the temperature is 20°C, and the humidity is above 75%, cultiv...

Embodiment 3

[0039] (1) Take fresh root tissue of Cruciferous plants infected by Plasmodium brassicae and crush it, filter it through gauze with a pore size of 25 μm, and centrifuge the filtrate at a speed of 3000 r / min for 9 minutes to remove Supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant, then add an appropriate amount of distilled water to the sediment, centrifuge at a speed of 4000r / min for 12min, remove the supernatant solution, and then distilled water was added to the precipitate to suspend the precipitate until 1×10 7 CFU / mL of Plasmodium brassicae bacteria liquid, stored at 4°C for later use;

[0040] (2) Sow sterilized cruciferous seeds in the sterilized quartz sand, soak the quartz sand and seeds with MS basic culture solution, and put them under the light intensity of 100μmolphotons / (m 2 .s), light time of 17h, temperature of 21°C, and humidity above 75%, cultivate until the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for cultivating cruciferae plants for observing the plasmodiophoromycetes infection. Root organizations of the initially-pathogenetic cruciferae plants are taken to prepare a plasmodiophoromycetes solution, cruciferae seeds are sowed in quartz sand, and the quartz sand and the seeds are soaked with an MS basic culture solution; under the conditions that the illumination intensity is 100 micro-mol photons/(m<2>.s), the illumination time ranges from 16 h to 18 h, the temperature ranges from 20 DEG C to 22 DEG C, and the humidity is higher than 75%, the seeds are cultivated until seed germination and are grown into plants with two main leaves. The plasmodiophoromycetes solution is applied to the roots of the plants to conduct the infection, and then root systems of the infected plants are observed through a biological microscope in a grading mode until the infection stage is finished. The method is easy and convenient to operate, distinct in observation result, small in occupied space and low in test cost, and has the advantages of being safe in operation, simple, high in repeatability, convenient to observe and distinct in result.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner 云南省农业科学院园艺作物研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com