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Method for extracting and separating low viscosity tremella aurantialba polysaccharide

A technology for the separation and separation of Auricularia polysaccharides, which is applied in the field of deep processing of edible fungi, can solve problems such as stickiness, difficulty in extraction and separation of Auricularia polysaccharides, foaming, etc., and achieve the effects of reducing viscosity, high health value, and reducing foam

Active Publication Date: 2014-07-16
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But Auricularia polysaccharide is a macromolecular compound, and it is reported that the polysaccharide molecular weight of Auricularia fruiting body is about 10 5 ~10 6 , when we extract Auricularia polysaccharides, the extract obtained with water as a solvent, as long as it is concentrated until the concentrated solution contains 2%-3% Auricularia polysaccharides, the solution is already quite viscous and foaming, which is suitable for the extraction of Auricularia polysaccharides. separation caused great difficulty

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Golden ear soaking solution: the dried golden ear is coarsely powdered into 20 meshes, soaked in a buffer solution with a pH value of 7.3 under stirring conditions, and the soaking time is 10 hours to obtain a golden ear soaking solution, a buffer solution with a pH value of 7.3 The weight ratio of liquid to 20-mesh golden ear dry product is 50:1, and the buffer solution with a pH value of 7.3 is a buffer solution containing 0.197% (percentage by weight) of disodium hydrogen phosphate and 0.022% (percentage by weight) of potassium dihydrogen phosphate. liquid.

[0014] (2) Ultrasonic treatment: heat the golden ear soaking solution to 40°C, and perform ultrasonic treatment. The ultrasonic intensity is 600W, and the treatment time is 20 minutes.

[0015] (3) Extraction of polysaccharides: heating the ultrasonically treated golden ear soaking solution to 90° C., extracting for 2 hours, and hot filtering the extracting solution to obtain a polysaccharide extracting solu...

Embodiment 2

[0019] (1) Golden ear soaking solution: The dried golden ear is coarsely powdered into 40 meshes, soaked in a buffer solution with a pH value of 7.3 under stirring conditions, and the soaking time is 8 to 10 hours to obtain the golden ear soaking solution, the pH value is 7.3 The weight ratio of the buffer solution to the 40-mesh golden ear dry product is 40:1, and the buffer solution with a pH value of 7.3 contains 0.197% (percentage by weight) of disodium hydrogen phosphate and 0.022% (percentage by weight) of potassium dihydrogen phosphate buffer.

[0020] (2) Ultrasonic treatment: heat the golden ear soaking solution to 50°C, and perform ultrasonic treatment. The ultrasonic intensity is 400W, and the treatment time is 30 minutes.

[0021] (3) Extraction of polysaccharides: heating the ultrasonically treated golden ear soaking solution to 95° C., extracting for 1.5 hours, and hot filtering the extracting solution to obtain a polysaccharide extracting solution.

[0022] (4)...

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PUM

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Abstract

The invention discloses a method for extracting and separating low viscosity tremella aurantialba polysaccharide, belonging to the technical field of deep processing of edible mushrooms. The method aims at overcoming the difficulty in extraction and separation of tremella aurantialba polysaccharide due to large viscosity and many bubbles of the tremella aurantialba polysaccharide solution in a tremella aurantialba polysaccharide extraction and separation process. The method uses degradation of high power ultrasonic on polysaccharide, and ultrasonic treatment is carried out under the condition of using specific buffer extracting solution with pH value of 7.3, so that the viscosity of the tremella aurantialba polysaccharide extracting solution is remarkably lowered, bubbles of the tremella aurantialba polysaccharide extracting solution are reduced, and the method has strong operability.

Description

technical field [0001] The invention belongs to the technical field of edible fungus deep processing, and in particular relates to a method for extracting and separating low-viscosity edible fungus polysaccharides. Background technique [0002] golden ear ( Tremella aurantiallba ) is a precious edible fungus with both medicinal and edible uses, rich in fungal polysaccharides with biological activity. As a food, it has the functions of immune enhancement and regulation, anti-radiation, anti-aging, etc.; as a raw material of cosmetics, because of its moisturizing and anti-radiation functions, it can play a role in skin rejuvenation and beauty, and maintain youth and fitness. But Auricularia polysaccharide is a macromolecular compound, and it is reported that the polysaccharide molecular weight of Auricularia fruiting body is about 10 5 ~10 6 , when we extract Auricularia polysaccharides, the extract obtained with water as a solvent, as long as it is concentrated until the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 高观世吴素蕊邰丽梅桂明英侯坤侯波
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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