Method of preparing natural milk-flavored essence by virtue of enzymatic hydrolysis of double enzymes and fermentation of multiple microorganisms
A technology of microbial fermentation and double-enzyme enzymatic hydrolysis, which is applied in the field of flavor preparation, can solve the problems of low fermentation efficiency, bad flavor, and low acid production, so as to achieve rich and rich milk flavor, improve flavor and taste, and enhance the sense of richness. Effect
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[0029] The specific operation steps of the present invention are described below in conjunction with specific embodiments, but the present invention is not limited to this embodiment:
[0030] a. Melting of raw cream: Melt bright cream at 30℃;
[0031] b. Add non-aqueous medium: use acetone as the medium and add 5 parts of acetone to 90 parts of cream;
[0032] c. Add emulsifier: add 0.1% monoglyceride;
[0033] d. Homogeneous emulsification: the homogenizer rotates at 10,000rpm and the emulsification time is 3min;
[0034] e. Pasteurization: 85°C for 5min;
[0035] f. Cooling down: cooling down to 50°C;
[0036] g. Double enzyme digestion: add Novozymes lipase Palatase 20,000L and Novozymes compound flavor protease at the same time for enzymolysis
[0037] g.1 Add enzyme amount: lipase is 430U / g substrate, protease is 330U / g substrate,
[0038] g.2 The temperature is 45℃,
[0039] g.3 Adjust the pH to 7.5,
[0040] g.4 Enzymolysis time 4h;
[0041] h. Enzyme inactivation: 15min at 85℃...
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