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A kind of preparation method of organic material decomposing agent

A technology of organic materials and decomposing agent, applied in the biological field, can solve the problems of high production cost, cumbersome use method, complicated preparation process, etc., and achieve the effect of increasing the composting temperature

Active Publication Date: 2016-02-17
DONGGUAN BAODE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to propose a decomposing agent for organic materials with low cost, simple process, convenient use and remarkable effect in view of the problems of high production cost, complicated preparation process, cumbersome use method and unstable application effect of the existing decomposing bacterial agent technology. Preparation

Method used

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  • A kind of preparation method of organic material decomposing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] A preparation method of an organic material decomposing agent comprises the following steps.

[0118] (1) Strain selection and breeding

[0119] A1, strain selection: select Bacillus subtilis, Bacillus licheniformis bacillus, Trichoderma konshii, Phanerochaete chrysosporium, and Sporiochaete thermophiles to form a group of bacterial strain combinations;

[0120] A2. Biosafety identification of strains: According to the general biosafety technology of microbial fertilizers, the safety appraisal of each strain in the above strain combination is carried out separately, and toxic and unsafe strains are removed.

[0121] (2) Preparation of seed solution

[0122] B1, slant culture

[0123] B11. Preparation of slant medium for each strain in the strain combination

[0124] Bacillus subtilis slant medium-beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, adjust the pH to neutral, and sterilize;

[0125] Bacillus licheniformis slant medium-beef extrac...

Embodiment 2

[0163] A preparation method of an organic material decomposing agent comprises the following steps.

[0164] (1) Strain selection and breeding

[0165] A1, strain selection: select Bacillus subtilis, Bacillus licheniformis bacillus, Trichoderma konshii, Phanerochaete chrysosporium, and Sporiochaete thermophiles to form a group of bacterial strain combinations;

[0166] A2. Biosafety identification of strains: According to the general biosafety technology of microbial fertilizers, the safety appraisal of each strain in the above strain combination is carried out separately, and toxic and unsafe strains are removed.

[0167] (2) Preparation of seed solution

[0168] B1, slant culture

[0169] B11. Preparation of slant medium for each strain in the strain combination

[0170] Bacillus subtilis slant medium-beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, adjust the pH to neutral, and sterilize;

[0171] Bacillus licheniformis slant medium-beef extrac...

Embodiment 3

[0209] A preparation method of an organic material decomposing agent comprises the following steps.

[0210] (1) Strain selection and breeding

[0211] A1, strain selection: select Bacillus subtilis, Bacillus licheniformis bacillus, Trichoderma konshii, Phanerochaete chrysosporium, and Sporiochaete thermophiles to form a group of bacterial strain combinations;

[0212] A2. Biosafety identification of strains: According to the general biosafety technology of microbial fertilizers, the safety appraisal of each strain in the above strain combination is carried out separately, and toxic and unsafe strains are removed.

[0213] (2) Preparation of seed solution

[0214] B1, slant culture

[0215] B11. Preparation of slant medium for each strain in the strain combination

[0216] Bacillus subtilis slant medium-beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, adjust the pH to neutral, and sterilize;

[0217] Bacillus licheniformis slant medium-beef extrac...

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Abstract

The invention relates to the technical field of agriculture microbial agent preparation processes and particularly relates to a method for preparing a commercialized organic material decomposition agent. The method comprises the following steps of: (1) selecting and breeding a strain and identifying the safety of the strain; (2) preparing a seed solution, including preparing a seed solution, a culture medium and a secondary seed solution; (3) carrying out stack-retting fermentation, including setting a stack-retting fermentation culture medium formula and fermentation conditions of the formula; and (4) drying, powdering, metering and packaging a stack-retting product, thereby preparing the organic material decomposition agent. According to the method, the production cost is low, the process is simple and mature, the use is convenient, and the decomposition application effect is remarkable, so that the method is beneficial to popularization and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of an organic material decomposing agent. Background technique [0002] In the prior art, the core microorganisms of the decomposing agent for organic materials are generally subjected to liquid or solid fermentation according to the difference in microbial classification level, and then the products of the microbial agent are mixed, carrier adsorbed, and dried according to a certain ratio. [0003] At present, most of the decomposing agent products are a new product of organic material decomposing agent through the expansion of compound bacterial strains, mixed fermentation or single fermentation and simple mixing, and it does not involve the evaluation of organic material decomposing agent The commercial areas involved in the indicators, such as implementation standards, quality supervision, and field demonstration and promotion, lack the exploration of mass pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12R1/125C12R1/10C12R1/885C12R1/645C12R1/145C12R1/01
CPCY02W30/40
Inventor 张新雄王亚君毛光平张东升
Owner DONGGUAN BAODE BIOLOGICAL ENG
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