Function and application of dickkopf-3 (dkk3) gene in coronary atherosclerotic heart disease
A technology for coronary atherosclerosis and heart disease, applied in gene therapy, cardiovascular system diseases, pharmaceutical formulations, etc., can solve problems such as inability to effectively and completely repair myocardial tissue, death, arrhythmia, etc.
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Embodiment 1
[0028] [Example 1] Myocardial infarction (MI) model acquisition
[0029] 1. Grouping of experimental animals: male C57BL / 6 background mice (DKK3+ / +), DKK3 knockout mice (DKK3- / -), heart-specific DKK3 transgenic mice (DKK3-TG) and non-transgenic mice ( NTG), a myocardial infarction (MI) model was induced by ligation of the left anterior descending coronary artery (LAD) of the mouse heart. They were randomly divided into 8 groups, each group: C57BL / 6 background a-MHC-Cre mouse sham operation group (DKK3+ / +Sham) and MI operation group (DKK3+ / +MI), DKK3 gene knockout mouse sham operation group ( DKK3- / -Sham) and MI operation group (DKK3- / -MI), non-transgenic mouse sham operation group (NTGSham) and MI operation group (NTGMI), heart-specific DKK3 transgenic mouse sham operation group (DKK3-TGSham ) and MI group (DKK3-TGMI).
[0030] 2. The MI model uses blocking the left anterior descending coronary artery (LAD) of the mouse heart to cause myocardial infarction. The operation pro...
Embodiment 2
[0039] [Example 2] Detection of myocardial infarction ratio, myocardial hypertrophy and fibrosis in mouse myocardial infarction (MI) model
[0040] 1. Take materials
[0041] (1) Preliminary work: prepare a urine cup filled with 20mL of 10% formaldehyde in advance, and label it (mouse number, group, type of operation and date of collection). Place the petri dish filled with 10% KCl solution at the sampling site. Turn on the analytical balance, adjust to zero, and then weigh and kill the mice.
[0042] (2) Material collection: Ophthalmic curved forceps clamped the vascular pedicle below the atrial appendage, cut off the heart, and quickly placed it in 10% KCl solution. After the heart stops beating in the diastolic phase, place it on sterilized gauze, gently squeeze the fluid in the heart cavity, dip the surface fluid dry, weigh and record, put the heart into the corresponding urine cup, fix it for 48 hours and use it for pathological testing.
[0043] (3) Relevant measurem...
Embodiment 3
[0063] [Example 3] Detection of cardiac function in myocardial infarction (MI) model mice
[0064]1 Ultrasound detection of cardiac function
[0065] 1.1 Preliminary preparation
[0066] (1) Anesthesia machine preparation: first connect the oxygen cylinder and the air inlet port on the anesthesia machine, then unscrew the sealing cap of the dosing port on the anesthesia machine, quickly add isoflurane to the safety scale, and then tighten the sealing cap. Unscrew the main valve on the oxygen cylinder, adjust the knob of the flow control valve, and maintain the outlet pressure at 0.2-0.3mPa.
[0067] (2) Preparation of the mice to be tested: After the mice to be tested were quickly anesthetized with isoflurane, the hair on the left chest area was shaved, and the head of the treated mice was inserted into the anesthetic catheter sleeve, and treated with 1.5-2.0% isoflurane Alkane maintains a stable anesthesia in mice.
[0068] 1.2 Cardiac function test
[0069] The mice were...
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