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A method for promoting glut4 gene expression in muscle cells

A muscle cell, gene expression technology, applied in the field of promoting GLUT4 gene expression, to achieve the effect of improving absorption, promoting glycogen anabolism, improving blood sugar metabolism rate and insulin sensitivity

Active Publication Date: 2016-09-07
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the effect of 4-PBA on the gene expression of GLUT4 protein

Method used

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  • A method for promoting glut4 gene expression in muscle cells
  • A method for promoting glut4 gene expression in muscle cells
  • A method for promoting glut4 gene expression in muscle cells

Examples

Experimental program
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specific Embodiment approach 1

[0028] Specific embodiment one: A kind of method of promoting the expression of GLUT4 gene in the muscle cell of the present embodiment is carried out according to the following steps: the muscle cell is subjected to induction differentiation culture, on the seventh day of induction differentiation culture, to induction differentiation medium After adding 1-10mmol / L 4-PBA in the medium, the cell culture condition is 37℃, CO 2 Cultured under the condition of 5% volume concentration, the culture time is 12-24 hours, and then the cells are recovered, and the method for promoting the expression of GLUT4 gene in muscle cells is completed.

[0029] The 4-PBA of this embodiment was purchased from Sigma. The muscle cells of this embodiment are commercially available.

[0030]The mechanism of action of 4-PBA of this embodiment is to inhibit HDAC activity. 4-PBA can attenuate endoplasmic reticulum stress and improve blood glucose metabolic rate and insulin sensitivity in diabetic mice...

specific Embodiment approach 2

[0031] Embodiment 2: This embodiment differs from Embodiment 1 in that: the amount of 4-PBA added is 1-8 mmol / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0032] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the amount of 4-PBA added is 2-7 mmol / L. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a method for promoting GLUT4 gene expression in a muscle cell, relates to a method for promoting GLUT4 gene expression, aims to build a method for effectively improving GLUT4 gene expression, and particularly relates to a method for promoting GLUT4 gene expression in the muscle cell to promote glucose absorption of the muscle cell. The method comprises the following steps: firstly, performing induction and differentiation culture on a muscle precursor cell (C2C12) so as to enable the muscle precursor cell to be cultivated to a mature muscle cell, then adding 4-PBA with the concentration of 1-10 mol / L into a culture medium, culturing for 12-24 h under the condition that the temperature is 37 DEG C and the volume concentration of CO2 is 5%, and recycling the cell; then detecting GLUT4 gene expression of the muscle cell and glucose absorption change of the muscle cell. The method provided by the invention is applied to the field of scientific research and clinical application for reducing diabetes hyperglycemia.

Description

technical field [0001] The invention relates to a method for promoting the expression of GLUT4 gene. Background technique [0002] 4-phenylbutyrate (4-phenylbutyrate, 4-PBA) is a small molecular compound with the molecular formula C 10 h 12 o 2 , with a molecular weight of 164 (Formula 1.1). FDA has approved 4-PBA to be used in clinical trials, but currently 4-PBA is only used as a special ammonia scavenger in clinical trials of human urea cycle disorders. In the process of treating urea cycle disorders, 4-PBA acts as a precursor of phenyl acetate to form phenyl acetate through β-oxidation. Phenyl acetate can be combined with glutamine to form a conjugate, phenylacetylglutamine, which converts blood ammonia, which is harmful to the human body, into a harmless compound, and is directly excreted out of the body through urine. In addition to being used as an ammonia scavenger, 4-PBA is often used as a sirtuin inhibitor and an endoplasmic reticulum stress inhibitor in the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
Inventor 顾宁胡海龙毛轲郭倩
Owner HARBIN INST OF TECH
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