Method for promoting GLUT4 gene expression in muscle cell
A muscle cell and gene expression technology, applied in the field of promoting GLUT4 gene expression
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specific Embodiment approach 1
[0028] Specific embodiment one: A kind of method of promoting the expression of GLUT4 gene in the muscle cell of the present embodiment is carried out according to the following steps: the muscle cell is subjected to induction differentiation culture, on the seventh day of induction differentiation culture, to induction differentiation medium After adding 1-10mmol / L 4-PBA in the medium, the cell culture condition is 37℃, CO 2 Cultured under the condition of 5% volume concentration, the culture time is 12-24 hours, and then the cells are recovered, and the method for promoting the expression of GLUT4 gene in muscle cells is completed.
[0029] The 4-PBA of this embodiment was purchased from Sigma. The muscle cells of this embodiment are commercially available.
[0030]The mechanism of action of 4-PBA of this embodiment is to inhibit HDAC activity. 4-PBA can attenuate endoplasmic reticulum stress and improve blood glucose metabolic rate and insulin sensitivity in diabetic mice...
specific Embodiment approach 2
[0031] Embodiment 2: This embodiment differs from Embodiment 1 in that: the amount of 4-PBA added is 1-8 mmol / L. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0032] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the amount of 4-PBA added is 2-7 mmol / L. Others are the same as in the first or second embodiment.
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