Sporolactobacillus terrae and application thereof

A technology of Lactobacillus geosporum and Lactobacillus sp., which is applied in the field of D-lactic acid preparation, can solve the problems of long fermentation time and low concentration of D-lactic acid produced, and achieves the effects of low cost, simplified operation process and cost saving.

Active Publication Date: 2015-07-15
SHANGHAI JIAO TONG UNIV
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These disclosed technologies, compared with L-lactic acid producing strains and methods, D-lactic acid producing bacterial strains and methods have the shortcomings of too long fermentation time or relatively low D-lactic acid concentration, and further development is needed based on efficient D-lactic acid producing bacterial strains production method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sporolactobacillus terrae and application thereof
  • Sporolactobacillus terrae and application thereof
  • Sporolactobacillus terrae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Production of D-lactic acid by batch fermentation with Lactobacillus geobacillus (HKM-1) using glucose as carbon source and dry corn steep liquor as nitrogen source in Erlenmeyer flasks

[0072] The composition of each culture medium used in the present embodiment is as follows:

[0073] Each liter of the slant medium contains: 30-50 g of glucose, 5-10 g of yeast powder, 2-8 g of peptone, 50 g of calcium carbonate, 15-25 g of agar powder, and the balance is water. The pH of the slant medium was 6.0. It was sterilized at 115° C. for 15 minutes.

[0074] Each liter of the seed medium contains: 40-120 g of glucose, 5-10 g of yeast powder, 2-8 g of peptone, 50 g of calcium carbonate, and the balance is water. The pH of the seed medium is 5.0-7.0. It is sterilized at 115° C. for 15 minutes.

[0075] Each liter of the fermentation medium contains: 60-120g of glucose, 20g of corn steep liquor dry powder, 50g of calcium carbonate, and water as the balance; the pH of the ferme...

Embodiment 2

[0085] Lactobacillus geobacillus (HKM-1) was used to produce D-lactic acid by batch fermentation in a conical flask with sucrose as carbon source and dry corn steep liquor as nitrogen source:

[0086] The slant medium and seed medium are the same as in Example 1.

[0087] Each liter of the fermentation medium contains: 60-120g of sucrose, 20g of corn steep liquor dry powder, 50g of calcium carbonate, and water as the balance; the pH of the fermentation medium is 5.0-7.0. It is sterilized at 115°C for 15 minutes.

[0088] The method for producing D-lactic acid by fermentation comprises the following steps:

[0089] (1) Incline cultivation: same as Example 1;

[0090] (2) Seed culture: the same as in Example 1;

[0091] (3) Fermentation culture: Put 5mL of the seed culture solution prepared in step (2) into a 100mL Erlenmeyer flask filled with 40mL of fermentation medium, and culture it statically at 42°C. When the rate of change of D-lactic acid is 0, it is regarded as The f...

Embodiment 3

[0097] Lactobacillus geobacillus (HKM-1) was used to produce D-lactic acid by static fermentation in a Erlenmeyer flask with maltose as carbon source and dry corn steep liquor as nitrogen source at 42°C. Fermentation was terminated when the glucose and D-lactic acid levels remained stable.

[0098] The composition of each culture medium used in the present embodiment is as follows:

[0099] The slant medium and seed medium are the same as in Example 1.

[0100] Each liter of the fermentation medium contains: 60-120g of maltose, 20g of corn steep liquor dry powder, 100g of calcium carbonate, and water as the balance; the pH of the fermentation medium is 5.0-7.0, and it is sterilized at 115°C for 15 minutes.

[0101] The method for producing D-lactic acid by fermentation comprises the following steps:

[0102] (1) Incline cultivation: Same as Example 1.

[0103] (2) Seed culture: same as in Example 1.

[0104] (3) Fermentation culture: Put 5mL of the seed culture solution pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
optical purityaaaaaaaaaa
Login to view more

Abstract

Provided are sporolactobacillus terrae (sporolactobacillus terrae) HKM-1s with collection numbers CCTCCNO: M2013389 and DSMNo.27555, and a method for producing D-lactic acid therefrom by means of fermentation of the same. The sporolactobacillus terrae HKM-1s can be used for producing D-lactic acid by using a nitrogen source, namely, dry powder of corn steep liquor; the highest fermentation yield of the D-lactic acid is 197g / L, the corresponding fermentation period is 47h, and the fermentation production capacity reaches 4.19g / L / h, and the optical purity of the produced D-lactic acid is 99.9%.

Description

technical field [0001] The invention discloses a bacterial strain in the technical field of D-lactic acid preparation and its application method, specifically a kind of Lactobacillus terreus sporogenes using cheap nitrogen source-corn steep liquor dry powder as an organic nitrogen source to prepare D-lactic acid with high optical purity and high concentration and its application method. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, can be divided into D-lactic acid, L-lactic acid and DL-lactic acid due to different optical activity. Its production methods mainly include chemical synthesis and microbial fermentation. The chemical method can only synthesize DL-lactic acid, while the fermentation method can synthesize a single L-lactic acid, D-lactic acid or DL-lactic acid according to the different strains used. Currently, about 90% of lactic acid is produced by microbial fermentation. Since the human body only has L-lactate dehydrogenase ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/01
CPCC12P7/56C12N1/205C12R2001/01
Inventor 许平黄开明唐鸿志陶飞
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap