Olaquindox metabolite immunochromatography test paper card and preparation method thereof

An immunochromatographic test paper and metabolite technology, which is applied in the field of olaquindox metabolite immunochromatographic test paper card and its preparation, can solve the problems of inability to operate on-site, high detection cost, complicated processing, etc., and achieve strong specificity and high sensitivity , the effect of simple operation

Inactive Publication Date: 2014-01-08
HENAN INST OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The residual detection methods of olaquindox and its metabolites mainly include thin-layer chromatography, high-performance liquid chromatography, mass spectrometry, high-performance liquid chromatography-mass spectrometry, etc. These methods are sensitive, accurate, specific, and have good resolution. Expensive instruments and equipment, complex sample pretreatment, tedious and time-consuming, high detection cost...

Method used

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  • Olaquindox metabolite immunochromatography test paper card and preparation method thereof
  • Olaquindox metabolite immunochromatography test paper card and preparation method thereof
  • Olaquindox metabolite immunochromatography test paper card and preparation method thereof

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Embodiment

[0021] Preparation of detection antigen

[0022] Accurately weigh 40 mg of 3-methylquinoxaline-2-carboxylic acid (MQCA), 12 mg of N-hydroxysuccinimide (NHS), 45 mg of N,N-dicyclohexylcarbodiimide (DCC) mg, dissolved in 2 ml of 1,4-dioxane solution, stirred at 37 ℃ for 12 h in the dark, and centrifuged to remove the precipitate, called A solution. Dissolve 20 mg of OVA in 5 ml of carbonate buffer solution (CBS, 0.05 mol / L, pH 9.6), called solution B. Add liquid A dropwise to liquid B under stirring condition, shake and react at 4 ℃ overnight. After the reaction was completed, it was centrifuged, and the supernatant was dialyzed with PBS buffer solution for 6 days, and the prepared detection antigen MQCA-OVA was freeze-dried and stored. The detection antigen synthesis route is shown in the following reaction equation.

[0023]

[0024]

[0025] Preparation method of specific monoclonal antibody against olaquindox metabolite

[0026] 1. Artificial antigen synthesis: the a...

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Abstract

The invention discloses an olaquindox metabolite immunochromatography test paper card and a preparation method thereof and belongs to the technical field of veterinary drug residue immunochemistry fast detection. The test paper card comprises a plastic card shell and a test paper strip encapsulated in the plastic card shell. A sample adding hole and an observation window are formed in the plastic card shell. The bottom layer of the test paper strip is a support layer. A sample pad, a colloidal gold pad, a testing pad and a water absorption pad which are connected in a overlap manner are sequentially attached to the support layer from left to right. The test paper card is simple to operate, fast, accurate, high in flexibility, high in specificity, vivid and visualized in result display, and the like. In addition, the test paper card is capable of on-site monitoring and suitable for screening of a huge amount of samples.

Description

technical field [0001] The invention belongs to the technical field of immunochemical rapid detection of veterinary drug residues, and in particular relates to a olaquindox metabolite immunochromatographic test paper card and a preparation method thereof. Background technique [0002] Olaquindox (Olaquindox), also known as quinamide alcohol and fast Yuling, is a feed additive developed and synthesized by West German Bayer Pharmaceutical Factory in the early 1970s. Because of its broad-spectrum antibacterial effect and growth-promoting effect, it is widely used in Livestock and poultry breeding, antibacterial treatment and disease prevention and control of aquatic animals. However, a large number of biological toxicity tests show that: excessive olaquindox will remain in the animal body, which has obvious cumulative toxicity, carcinogenicity, mutagenesis and DNA damage and other toxic side effects. In view of the harm to human health, the EC2788 / 98 resolution of the European...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 张慧辉姜金庆王自良李广领杨雪峰范国英杨理赵旭娜刘长忠
Owner HENAN INST OF SCI & TECH
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