Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense

A technology of molecular markers and primer sets, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of screening polymorphic microsatellite markers without reports

Inactive Publication Date: 2015-06-03
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the freshwater shrimp EST database contains a large number of EST sequences, and it has been reported that multiple genes related to sex, reproduction, and development have been cloned from these sequences, but the method of screening polymorphic microsatellite markers from the freshwater shrimp EST library has not been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] Screening of Microsatellite Sequences and Determination of Polymorphic Markers

[0031] 1. Microsatellite sequence source and screening

[0032] The screening method of EST-SSR marker-specific primers for freshwater shrimp includes the following steps: the green shrimp androgenic gland transcriptome library (http: / / www.ffrc.cn / gene / list.asp) constructed by our research group. Among the shrimp ESTs, the library sequences are all the EST sequences of the freshwater shrimp, which are derived from the androgenic gland of the freshwater shrimp. The microsatellite sequence retrieval software SSR-hunter was used to search the microsatellite sequence, and a total of 70,702 EST sequences were used. For 2-6 Microsatellite sequences with repeats greater than 5 base repeat units were isolated. (According to the principle of microsatellite screening, the most basic principle is: the number of repetitions of 2 and 2 upper base repeat units is not less than 5 times. On this basis, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of DNA marker technology and application of molecular biology, in particular to the screening and application of EST microsatellite marker (EST-SSR) of freshwater economic crustacean freshwater shrimp. The EST-SSR marker technology can be used to study the genetic characteristics and population structure of the freshwater shrimp breeding population, realize the preliminary application of molecular markers in freshwater shrimp breeding, and carry out molecular research for the construction of high-density green shrimp genetic linkage pictures and QTL positioning of important economic traits. Marker-assisted breeding lays the foundation.

Description

technical field [0001] The invention belongs to the field of molecular biology DNA marker technology and application, in particular to the freshwater economic crustacean green shrimp (Macrobrachium japonicus, Macrobrachium nipponense ) Screening and application of EST (expressed sequence tags, ESTs) microsatellite markers (EST-SSR). Background technique [0002] Freshwater shrimp, scientific name Japanese river prawn ( Macrobrachium nipponense ), belonging to the order Decapoda, the family Palaemonidae, and the genus Macrobrachium, widely distributed in China, Japan, Korea, Vietnam, Myanmar and other Asian countries, with high nutritional value, fast growth, and adaptability Strong and other characteristics, deeply welcomed by consumers, is an important economic farming shrimp. At present, 18 provinces and cities (districts) in my country are cultivating freshwater shrimp, and its aquaculture area ranks first in freshwater shrimp. According to the 2011 statistics of "Chin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 乔慧傅洪拓周巧龚永生蒋速飞熊贻伟金舒博张文宜
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com