Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense

A molecular marker and primer set technology, which is applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., and can solve the problem of unreported screening of polymorphic microsatellite markers.

Inactive Publication Date: 2014-01-01
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the freshwater shrimp EST database contains a large number of EST sequences, and it has been reported that multiple genes related to sex, reproduction, and development have been cloned from these sequences, but the method of screening polymorphic microsatellite markers from the freshwater shrimp EST library has not been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense
  • Primer group, marking method and application of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeats) molecular marker of macrobrachium nipponense

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] Screening of Microsatellite Sequences and Determination of Polymorphic Markers

[0036] 1. Microsatellite sequence source and screening

[0037] The screening method of EST-SSR marker-specific primers for freshwater shrimp includes the following steps: the green shrimp androgenic gland transcriptome library (http: / / www.ffrc.cn / gene / list.asp) constructed by our research group. Among the shrimp ESTs, the library sequences are all the EST sequences of the freshwater shrimp, which are derived from the androgenic gland of the freshwater shrimp. The microsatellite sequence retrieval software SSR-hunter was used to search the microsatellite sequence, and a total of 70,702 EST sequences were used. For 2-6 Microsatellite sequences with repeats greater than 5 base repeat units were isolated. (According to the principle of microsatellite screening, the most basic principle is: the number of repetitions of 2 and 2 upper base repeat units is not less than 5 times. On this basis, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of molecular biological DNA (Deoxyribonucleic Acid) marking technologies and applications thereof and particularly relates to a sieving method and application of an EST (Expressed Sequence Tag) microsatellite marker (EST-SSR (Simple Sequence Repeats)) of a macrobrachium nipponense as a freshwater economic shelled animal. By using an EST-SSR marking technology, the hereditary feature and group structure of a macrobrachium nipponense breeding group can be researched, the primary application of a molecular marker in macrobrachium nipponense breeding can be realized, and a foundation is laid for carrying out auxiliary molecular marker breeding through constructing a high-density macrobrachium nipponense genetic linkage picture and locating the important economic character of a QTL (Quantitative Trait Locus).

Description

[0001] technical field [0002] The invention belongs to the field of molecular biology DNA marker technology and application, in particular to the freshwater economic crustacean green shrimp (Macrobrachium japonicus, Macrobrachium nipponense ) Screening and application of EST (expressed sequence tags, ESTs) microsatellite markers (EST-SSR). [0003] Background technique [0004] Freshwater shrimp, scientific name Japanese river prawn ( Macrobrachium nipponense ), belonging to the order Decapoda, the family Palaemonidae, and the genus Macrobrachium, widely distributed in China, Japan, Korea, Vietnam, Myanmar and other Asian countries, with high nutritional value, fast growth, and adaptability Strong and other characteristics, deeply welcomed by consumers, is an important economic farming shrimp. At present, 18 provinces and cities (districts) in my country are cultivating freshwater shrimp, and its aquaculture area ranks first in freshwater shrimp. According to the 2011 ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 乔慧傅洪拓周巧龚永生蒋速飞熊贻伟金舒博张文宜
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com