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A kind of red leaf marker restorer line and its three-line hybrid breeding method

A technology of restorer lines and hybrids, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problems of low land utilization rate and affect seed production yield, etc., and achieves comprehensive utilization value improvement and easy identification of true and false. , the effect of high clothing

Active Publication Date: 2016-04-27
山东黄河口宜信农业开发有限公司
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Problems solved by technology

The disadvantage of the two-line method is that 50% of the fertile plants need to be pulled out, and the land utilization rate is low, which affects the seed production yield

Method used

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  • A kind of red leaf marker restorer line and its three-line hybrid breeding method

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Embodiment

[0018] Select parent:

[0019] Yinmian No. 2: [Wang Yuan, Zhang Rui, Zu Maotang et al. Bivalent transgenic insect-resistant three-line hybrid cotton Yinmian No. 2, China Cotton, 2005, 32(9): 20], Yinmian No. A new three-line hybrid cotton variety with high yield, insect resistance and disease resistance jointly selected by the Institute of Biotechnology of the Academy of Agricultural Sciences, Handan Academy of Agricultural Sciences and Beijing Silver Land Biotechnology Co., Ltd. In 2003, it participated in the regional test of national cotton varieties. In 2005, this variety obtained the national transgenic biological safety certificate, and in March of the same year, it passed the examination and approval of the National Crop Variety Approval Committee.

[0020] Red leaf marker parent 96-67: [Ji Jiahua, Li Hongqin, Wang Ende, etc. Breeding and application of marker cotton parents, China Seed Industry, 2005, (10): 39-40], Table 1 lists typical marker stigmas Exposed germplas...

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Abstract

The invention relates to a red leaf marker restorer line and a method for breeding three-line hybrids thereof. In the method, Yinmian No. 2 is used as the female parent and the red leaf marker parent 96-67 is used as the male parent for crossbreeding and mating, and the F1 to F2 generations are planted. The fertile plants with red leaves were selected for generation and mixed harvest, and the F3 generation was planted. The fertile plants with red leaves were selected for the F3 generation, selfed for 4 consecutive generations, and the lines of the isolated sterile lines were eliminated, and 5 individual lines were selected from the red leaf lines finally selected. As the male parent, the cytoplasmic sterile line 4PB404A was pollinated to obtain hybrid bolls. After flowering, the pollination of the plants was checked, the recovery rate was calculated, and the red leaf marker restorer line 11HF106R with a recovery rate of 100% was selected and bred with 26 Cytoplasmic male sterile line was test crossed, and Lumianyan 28 was used as a control, and a marker-type three-line hybrid combination 25MA×11HF106R with good yield increasing effect was bred. The method overcomes the risk of sterile plants caused by natural outcrossing, improves the purity of varieties, ensures 100% utilization of heterosis, improves seed production yield, reduces seed production costs, and improves production efficiency.

Description

technical field [0001] The invention relates to the technical field of cotton breeding, in particular to a red leaf marker restorer line and a method for breeding three-line hybrids thereof. Background technique [0002] Heterosis is a common phenomenon in the biological world and has been widely used in crops such as grain and vegetables. Applying heterosis to cotton is an effective way to greatly increase cotton yield, improve cotton fiber quality and enhance the ability of cotton plants to resist diseases and insect pests. my country's cotton production and popularization practice shows that hybrid cotton varieties have a much larger yield increase than conventional cotton varieties. Generally, the first-generation hybrids of strong dominant upland cotton varieties have a 15%-30% increase in yield than conventional popularized varieties, and the second-generation hybrids also have a stronger The potential for increasing production can reach 5%-10%. [0003] At present, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 纪家华赵永民王义平
Owner 山东黄河口宜信农业开发有限公司
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