Water saving method in process of producing sodium gluconate through aspergillus niger fermentation
A sodium gluconate and Aspergillus niger fermentation technology, which is applied in the field of industrial biology, can solve the problems of large water resource consumption, and achieve the effects of saving water quality, reducing steam volume, and reducing steam consumption
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Embodiment 1
[0016] Embodiment 1: the production technology of aspergillus niger fermentation production sodium gluconate
[0017] Prepare a glucose solution with a concentration of 20-30% (w / v) with fresh water, then add appropriate amount of inorganic salt and other nutrients in proportion (conventional formula), and inoculate the liquid with a volume fraction of 5-12% black Aspergillus liquid seeds are subjected to conventional fermentation. During the fermentation process, 30-40% sodium hydroxide solution is added to control the pH to 5.5-7.0. When the glucose concentration in the feed liquid drops to 0-1g / L, the fermentation is ended to obtain 20-30% (w / v) sodium gluconate fermentation liquid, the fermentation period is 20-30 hours. Sterilize the fermentation broth through plate and frame filtration, decolorize the activated carbon, filter to remove the activated carbon, concentrate the clear liquid through multi-effect evaporation (produce a large amount of condensed water), cool dow...
Embodiment 2
[0018] Embodiment 2: Concentrating and evaporating the fermented liquid containing sodium gluconate, collecting the evaporative condensed water produced in the evaporation process, and implementing reverse osmosis treatment to it
[0019] After fermentation according to the method described in Example 1, the fermented liquid is heated to inactivate the thallus, then the mycelium is removed by plate and frame filtration, the fermentation clear liquid is decolorized by adding activated carbon, the activated carbon is removed by plate and frame filtration, and the clear liquid after decolorization is pumped to The four-effect evaporator concentrates, collects evaporative condensed water, that is, the condensed water generated by the concentration of the feed liquid (including the condensed water generated by heating steam (accounting for 1 / 5 of the total)), and pumps it to the high-temperature resistant reactor produced by GE in the United States. The osmotic membrane Duratherm ST...
Embodiment 3
[0020] Embodiment 3 Fresh water ingredients prepare the fermented liquid of Aspergillus niger fermentation to produce sodium gluconate
[0021] Dissolve an appropriate amount of glucose in fresh water (tap water, water temperature 20-25°C) according to the formula of the fermentation feed liquid, prepare the glucose concentration to 25-26%, and add other inorganic salt auxiliary materials at the same time, the feed liquid temperature is about 15-20°C, Sterilization is carried out by conventional sterilization methods. After cooling down, sodium gluconate production strains are inoculated, and fermentation is carried out according to conventional fermentation control conditions. The fermentation period is 24-27 hours.
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