Fungi strain of Tulasnella and application thereof in promotion of germination of Cymbidium mannii seeds

A technology of glue film bacteria and sclerophyllum, which is applied in the field of glue film fungus strains and its application in promoting the germination of sclerophyllum seeds, and can solve the problems such as no glue film bacteria

Active Publication Date: 2013-12-25
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on Tulasnella strain (CGMCC No.7553) and its use and method in promoting the growth of sclerophylla seeds to seedling growth in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0037] 1. Firstly, obtain the strain of Glycobacteria (preservation number: CGMCC No.7553):

[0038] The present invention isolates the CGMCC No.7553 strain from the protocorm produced by ex situ symbiosis germination: collect the litter, bark, moss and humus around the original plant of Sclerophyllum as culture substrate, and the seeds of Sclerophyllia Carry out ex situ symbiotic germination in the laboratory, induce seed germination to produce protocorms, sterilize the surface of the obtained protocorms and culture them with artificial medium under sterile conditions, induce the growth of endophytic fungi in the protocorms, and wait for the growth of fungi For silk, purify the fungus to obta...

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Abstract

The invention relates to a fungi strain [CGMCC (China General Microbiological Culture Collection Center) No.7553] of Tulasnella and application thereof for promoting Cymbidium mannii seeds to germinate, wherein the ITS (Internal Transcription Sequence) sequence of the fungi strain is submitted to the database of National Center of Biotechnology Information (NCBI, http: / / www.ncbi.nlm.nih.gov / ), and the Genbank number is KC796458.1. The strain (CGMCC) of Tulasnella forms a symbiotic relationship with the protocorm so as to promote the Cymbidium mannii seeds to germinate and grow into seedlings. Experiments verify that the seed germination rate of inoculating the strain (CGMCC No.7553) separated from the protocorm of the Cymbidium mannii is 74.72%+ / -16%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an effective fungal strain (Tulasnella) bacterial strain (CGMCC No. .7553), and its application in promoting the germination of sclerophyllum seeds, in particular an effective fungus capable of forming a symbiotic relationship with sclerophyllum protocorms and promoting the germination and growth of its seeds to the seedling stage. Background technique [0002] The seeds of orchids are very small, with only immature embryos. Under natural conditions, seed germination requires specific symbiotic fungi to obtain nutrients. Orchidaceae seeds can be germinated in two ways: non-symbiotic germination culture and symbiotic germination culture. At present, the germination of Orchidaceae seeds mostly uses artificial medium for non-symbiotic germination under sterile conditions. Although the germination rate is high, when endangered species are returned to the wild, the non-symbiotic germinatio...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H4/00C12R1/645
Inventor 邵士成高江云盛春玲
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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