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A method for simultaneous detection of chlorophyll content and biomass of Chlorella based on spectral technology

A chlorophyll content and spectroscopic technology is applied in the field of Chlorella chlorophyll content and biomass detection, which can solve the problems of cumbersome, time-consuming, labor-intensive operations, and achieve the effects of simplifying operation steps, shortening detection time, and rapid and accurate detection.

Active Publication Date: 2015-12-09
ZHEJIANG UNIV
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Problems solved by technology

[0006] The invention provides a method for simultaneous detection of chlorophyll content and biomass of chlorella based on spectral technology, which solves the problems of relatively cumbersome, time-consuming, and labor-intensive operations in existing detection methods

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  • A method for simultaneous detection of chlorophyll content and biomass of Chlorella based on spectral technology
  • A method for simultaneous detection of chlorophyll content and biomass of Chlorella based on spectral technology
  • A method for simultaneous detection of chlorophyll content and biomass of Chlorella based on spectral technology

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Embodiment Construction

[0036] The present invention is further explained below in conjunction with specific examples.

[0037] Chlorella samples were commercially available products, which were purchased from the Chinese Academy of Sciences Wildlife Germplasm Bank-Freshwater Algae Species Bank, product number FACHB-7.

[0038] Take a certain concentration of chlorella reaction solution 100ml, add water 0ml, 10ml, 20ml, 30ml, 40ml to dilute, and obtain five kinds of chlorella reaction solutions with different concentration gradients, 12 samples for each gradient, a total of 60 samples . Mix the reaction solution of each sample evenly, take 50ml of the chlorella reaction solution in it for spectrum collection, take each 8ml as a layer, collect the visible-near-infrared spectral transmittance value of each layer of solution, and calculate the transmittance of each layer average rate, figure 1 It is the average spectral transmittance figure of 60 samples of Chlorella obtained in this embodiment.

[0...

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Abstract

The invention discloses a method for detecting the chlorophyll content and the biomass of chlorella based on a spectrum technology. The method comprises the steps as follows: (1) collecting spectral transmittance values of a chlorella reaction liquid sample under the wavelengths of 439 nm, 543 nm, 696 nm and 1065 nm, and measuring the chlorophyll content of the sample; (2) creating a multiple linear regression model by taking the spectral transmittance values as input vectors and the measured chlorophyll content as an output vector, obtaining the biomass of the sample, and creating a correlation between the chlorophyll content and the biomass; and (3) collecting spectral transmittance values of chlorella-to-be-detected reaction liquid under the wavelengths, and substituting the spectral transmittance values into the model to work out the chlorophyll content and the biomass of the chlorella-to-be-detected reaction liquid. According to the method, the chlorophyll content and the biomass of the chlorella can be quickly and accurately detected, the operation steps are greatly reduced, the detection time is shortened, and consequences such as inaccurate measurement results caused by unskilled operation of an operator or subjective factors are also avoided.

Description

technical field [0001] The invention relates to the technical field of detection of chlorophyll content and biomass of chlorella, in particular to a method for simultaneously detecting the chlorophyll content and biomass of chlorella. Background technique [0002] Chlorella (Chlorella) is a kind of single-celled green algae, which belongs to Chlorophyta, Chlorophyceae, and Chlorella genus. It is widely distributed in nature and has the most species in freshwater waters. Chlorella is easy to cultivate and has a fast growth and reproduction speed. It can not only use light energy for self-support, but also use organic carbon sources for growth and reproduction under heterotrophic conditions. It has high application value. Studies have shown that chlorella is rich in protein, lipid, polysaccharide, dietary fiber, vitamins, trace elements and active metabolites. Scholars at home and abroad have conducted extensive research on the pharmacological effects of chlorella, and found ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 邵咏妮魏萱何勇
Owner ZHEJIANG UNIV
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