Fluorescence type glucose capillary biosensor

Abstract

The invention relates to a fluorescence type glucose capillary biosensor. The biosensor comprises nano sensors, a capillary coupling device, capillaries, a light source, a fiber optic spectrometer and a probe, wherein the nanometer sensors are distributed on the inner walls of the capillaries; the capillary coupler is connected with optical fibers and hoses; the optical fibers can be used for receiving an exciting light generated by the light source; the hoses can be used for receiving liquid to be detected; and a new fluorescence spectrum generated by the liquid to be detected and the nano sensors under the excitation of an evanescent field produced by the exciting light is received by the probe, and the probe transmits the received fluorescence spectrum to the fiber optic spectrometer to be analyzed. According to the fluorescence type glucose capillary biosensor, the capillaries are adopted for sampling, thus the dosage of samples to be detected can be obviously decreased, and reagents are saved. A fluorescence signal is excited through the evanescent field, therefore, the background fluorescence in a biological sample can be reduced efficiently, and the lower detection limit can be elevated.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis and quantification, in particular to a fluorescent glucose capillary biosensor, which can be applied to the concentration detection of trace glucose in trace samples. Background technique [0002] When light passes from an optically denser medium to an optically rarer medium, the angle of refraction is greater than the angle of incidence. When the incident angle increases to a certain angle, the refraction angle is equal to 90°. At this time, the refracted light disappears completely, and all the incident light returns to the original medium. This phenomenon is called total reflection. [0003] Experiments have shown that in total reflection, the light wave is not absolutely reflected back to the optically dense medium at the interface, but penetrates into a very thin surface of the optically sparse medium (about one wavelength) and propagates along the interface for some distance (wa...

AI Technical Summary

Problems solved by technology

[0006] 1. Conventional fluorescence detection based on the evanescent field adopts the method of separating the detection fiber probe from the solution to be tested. The amount of the solution to be tested is much larger than the effective sample volume actually involved in the concentration detection, so a large amount of reagents are wasted in conventional detection, and the experimental cost High, it is difficult to achieve trace detection and analysis of samples

[0007] 2. The operation process in conventional detection is not simple enough

[0008] 3. The amount of conventional detection solution is large, so other interference factors have a greater impact, resulting in detection sensitivity

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