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Method for preparing antigen to obtain antihydrophobic peptide antibody

一种疏水性、抗原的技术，应用在抗受体/细胞表面抗原/细胞表面决定因子免疫球蛋白、化学仪器和方法、抗动物/人类的免疫球蛋白等方向，能够解决小分子量等问题

Active Publication Date: 2013-09-11
TOAGOSEI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antigen must be of at least a certain molecular weight to meet these requirements, but peptides are usually of smaller molecular weight and metabolized rapidly after administration and therefore are not directly useful as antigens

Method used

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  • Method for preparing antigen to obtain antihydrophobic peptide antibody
  • Method for preparing antigen to obtain antihydrophobic peptide antibody
  • Method for preparing antigen to obtain antihydrophobic peptide antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0233] Antibody acquisition using antigen of the present invention (1), polyclonal antibody

[0234] Titers were measured by dot blot using serum obtained by direct immunization using a solution of SPAPP suspended in a solvent containing a nonionic surfactant as an immunogen.

[0235] (1) Antigen preparation - SPAPP / 1% Tween 20

[0236] Six (6) mg of SPAPP with a purity of 92% was weighed and suspended in 5.4 ml of pure water. At this point, SPAPP was completely insoluble in aqueous solution and became cloudy. The solution was left to stand in this state for about 3 hours, and 0.6 ml of 10% Tween 20 was added and mixed. The final concentration of SPAPP was 2 mg / ml. Ultrasonic waves were applied intermittently for a total of about 3 minutes by a TOMY SEIKO Handy Sonic Model UR-20P at power level 7. Ultrasonic vibrations were applied until the transparency increased to some extent and the absorbance at 600 nm reached about 0.2. The resulting SPAPP solution was aliquoted into ...

Embodiment 2

[0244] Particle size distribution and molecular weight distribution of SPAPP

[0245] (1) Measurement of particle size distribution

[0246] The particle size distribution of the SPAPP / 1% Tween 20 suspension used in the above immunization method was measured as follows. After mixing the SPAPP suspension (2 mg / ml) and an equal amount of 0.4% trypan blue solution, the solution was applied to a cell counting device Countess (registered trademark) (manufactured by INVITROGEN). Particles from 2 μm to 80 μm can be measured in this device.

[0247] (2) Therefore, the particle size distribution of SPAPP in the suspension is 2 to 60 μm, and the concentration is 3.2×10 6 / ml. Most have dimensions of a few μm. Figure 4 The measurement results of the molecular weight distribution are shown.

[0248] The molecular weight distribution of SPAPP aggregates in suspension was measured according to the following procedure. Figure 5 An outline of the process is displayed.

[0249] (i) Th...

Embodiment 3

[0260] Aggregate Molecular Size (Molecular Weight / Particle Size) Distribution Test

[0261] (1) The relationship between the standing time of aggregates in pure water and the molecular size distribution

[0262] The time from suspending SPAPP in pure water to adding various surfactants was changed, thereby confirming that the molecular size distribution of aggregates changed according to time. For example, in the case of 1% Tween 20, the insoluble fraction (precipitation) was 5% when the surfactant was added quickly after suspending SPAPP in pure water, 17% when added after 1.5 hours, and 17% when the surfactant was added after 1.5 hours. 48% when added after 3 hours.

[0263] (2) Molecular size distribution in other nonionic surfactant solutions

[0264] Check whether SPAPP forms high molecular weight aggregates in other nonionic surfactant solutions. PEG60 Lauryl Ether (Polypure), Triton X-100 and Nonil P-40 were used as surfactants at 1% each. As in the case of the meth...

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Abstract

The purpose of the present invention is to discover a method to obtain an antibody for hydrophobic peptides, which can be used for general purposes easily and with great reliability. This method for preparing an antigen is characterized in that hydrophobic peptides, which are not bonded to carrier proteins, are used as high molecular weight aggregates in an aqueous solution containing a nonionic surfactant.

Description

technical field [0001] The invention relates to a method for preparing an antigen in the field of immunoengineering. [0002] In particular, the present invention relates to a method for preparing an antigen for obtaining an anti-hydrophobic peptide antibody using a hydrophobic peptide as an immunogen without using a carrier protein. Background technique [0003] Antibodies have been used as very useful research reagents in many laboratories for decades due to their very specific molecular recognition ability and high affinity, and high probability of being easily produced in the target molecule to be analyzed, and It is actually used in a wide range of applications from diagnostic agents to pharmaceuticals. In many cases, the target of an antibody is a protein, but antibodies are also used for the highly sensitive detection of low molecular weight target compounds such as certain drugs and environmental pollutants. [0004] After completion of the analysis of the human ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C07K17/02G01N33/53
CPCC07K16/18C07K14/245C07K14/4711C07K14/70596C07K16/1232C07K16/2896G01N33/6896
Inventor 冈本雅次花村雅人福岛均吉田彻彦
Owner TOAGOSEI CO LTD
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