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Exosome-associated microRNA as a diagnostic marker

A biomarker and labeling technology, applied in the determination/inspection of microorganisms, coin-operated equipment for distributing discrete items, biological material analysis, etc.

Inactive Publication Date: 2013-09-04
UNIV OF LOUISVILLE RES FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The greatest difficulty in most current mass spectrometry methods is dynamic range, not sensitivity

Method used

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  • Exosome-associated microRNA as a diagnostic marker
  • Exosome-associated microRNA as a diagnostic marker
  • Exosome-associated microRNA as a diagnostic marker

Examples

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preparation example Construction

[0094] Biopolymer arrays (eg, polynucleotide microarrays) can be fabricated by depositing previously obtained biopolymers (eg, from synthetic or natural sources) on a substrate or by in situ synthetic methods. Methods for obtaining biopolymers by deposition include, but are not limited to: loading a needle or capillary and then contacting a surface, such as described in U.S. Patent 5,807,522; deposition by firing from a pulsed jet such as an inkjet head, such as PCT publication WO 95 / 25116 and WO 98 / 41531 and elsewhere. In situ fabrication methods include those described in US Pat. those methods for polynucleotides. Details of the fabrication of biopolymer arrays by precipitation of previously obtained biopolymers or by in situ methods are further described in US Pat. In fabricating arrays from biopolymers obtained by precipitation or by in situ methods, each area on the surface of a substrate on which an array is to be or has been formed (“array area”) is typically complet...

Embodiment 1

[0117] EPCAM-positive circulating exosomes in women with benign and malignant ovarian disease

[0118] EpCAM-positive exosomes were specifically isolated using magnetic beads of an anti-EpCAM antibody, and the total protein of these circulating exosomes was determined and plotted against the stage of the disease ( image 3 A). The level of EpCAM-positive exosomes in age-matched normal volunteers (controls) was 0.039 ± 0.030 mg / ml of exosomal protein, which represented the background value for the assay. Patients diagnosed with benign ovarian disease had 0.149 ± 0.065 mg / ml of exosomal protein, which was significantly higher than control values. Patients diagnosed with ovarian cancer all presented significantly higher levels of EpCAM-positive exosomes (compared with benign disease or controls). Women with stage I ovarian cancer presented 0.320±0.056 mg / ml of circulating exosomal protein, which was significantly higher than controls and benign disease (p image 3 B). The exoso...

Embodiment 2

[0120] Association of small RNAs with tumor-derived exosomes

[0121] To identify whether these isolated exosomes contain small RNAs, they were examined using Bio-Analyzer2100 ( Figure 4 ). These analyzes identified the presence of significant numbers of small RNAs in the absence of 18S and 28S RNAs that normally occur with cell-derived RNA. These materials were subsequently used for miRNA profiling.

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Abstract

The presently disclosed subject matter provides methods of diagnosis of cancer or adverse pregnancy outcomes in a subject by measuring amounts of one or more microRNAs present in cancer-derived exosomes isolated from a biological sample from the subject.

Description

[0001] This application is a divisional application of an invention patent application with an application date of July 25, 2008, an application number of 200880025224.0, and an invention title of "Exosome-associated microRNA as a diagnostic marker". [0002] related application [0003] This application claims priority based on U.S. Provisional Applications Serial No. 60 / 951,812, filed July 25, 2007, and U.S. Provisional Applications Serial No. 61 / 050,438, filed May 5, 2008, the entire disclosures of which are incorporated by reference way into this article. technical field [0004] The subject matter disclosed in this application relates to methods of diagnosis and prognosis of cancer and adverse pregnancy outcome. In particular, the subject matter disclosed herein relates to diagnostic and prognostic methods based on the determination in a biological sample from a subject of one or more exogenous The amount of microRNA (microRNA) in the body (exosome). Background techni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6809C12Q2600/112C12Q2600/178C12Q1/6886Y10T436/143333G07F17/3211G07F17/3258C12Q2563/161C12Q2525/207C12Q1/6813C12Q1/6883G01N33/574G01N33/57423G01N33/57449G01N33/57492C12Q2600/118C12Q2600/158
Inventor D·D·泰勒C·格塞欧-泰勒
Owner UNIV OF LOUISVILLE RES FOUND INC
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