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Molecular marking method as well as kit and primer for identifying purity of broccoli hybrid scarlet pimpernel variety

A technology of variety purity and molecular markers, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of not being intuitive enough, time-consuming and labor-intensive, etc., and achieve the effect of shortening the detection cycle and rapid identification

Inactive Publication Date: 2013-08-07
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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AI Technical Summary

Problems solved by technology

These detection methods are time-consuming, laborious and not intuitive

Method used

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  • Molecular marking method as well as kit and primer for identifying purity of broccoli hybrid scarlet pimpernel variety
  • Molecular marking method as well as kit and primer for identifying purity of broccoli hybrid scarlet pimpernel variety
  • Molecular marking method as well as kit and primer for identifying purity of broccoli hybrid scarlet pimpernel variety

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Embodiment Construction

[0034] In the following, the present invention will be further illustrated by the embodiment of SSR marker analysis based on PCR technology.

[0035] The experimental materials in this example are the commercial seeds of broccoli 'Hailu' and its parents, and 4 other broccoli varieties commonly used in production (excellent, Lvxiong 90, excellent cold resistance, magpie).

[0036] Methods: DNA was extracted from broccoli leaves, PCR was amplified using SSR molecular markers, the amplified products were subjected to gel electrophoresis, the results were counted and characteristic primers were screened.

[0037] 1. Genomic DNA Extraction from Broccoli

[0038] Genomic DNA of broccoli was extracted by conventional methods.

[0039] 2. Molecular marker analysis

[0040] The SSR reaction system is genomic DNA 20 ng, Mg 2+ 1.8 mM, dNTP 0.2 mM, primer 0.6 μM, 1X PCR buffer, Taq DNA polymerase 0.8 U, add sterile ultrapure water to 15 μL. The PCR amplification reaction was carried ou...

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Abstract

The invention discloses a primer for identifying purity of a broccoli hybrid scarlet pimpernel variety. The primer is a combination of one or more of four pairs of primers: (1), primers Ol12-D05; (2), primers Ra1-F06; (3), primers FITO439; and (4), primers Ol10-A11. The molecular marking method as well as the kit and the primer for identifying purity of broccoli hybrid scarlet pimpernel variety have the beneficial effects of being capable of clearly distinguishing and judging different varieties of broccoli and capable of accurately and quickly distinguishing the variety truth and variety purity, so that reliable technology is provided for variety identification, seed market management, variety protection, seed quality control before seeding, good seed breeding and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker method, a kit and primers for identifying the authenticity and or variety purity of broccoli hybrid 'Sea Green'. Background technique [0002] Excellent varieties are the basis for high crop yields, and mixed varieties and low purity will significantly reduce yield and commodity quality. Rapid and accurate identification of varieties and purity analysis play an important role in standardization of seed quality, variety approval, identification of fake species, and disputes over property rights. At present, my country's seed production and management are not standardized, unqualified seeds are repeatedly mixed into the market and used for production, resulting in reduced production and economic losses, so the identification of variety purity is particularly important (Wu Minsheng, Wang Shoucai, Dai Jingrui, DNA fingerprinting technology in Application of...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 虞慧芳顾宏辉王建升赵振卿盛小光陈纪算许映君
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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