Salt tolerance SyDBSP gene derived from synechocystis, and uses thereof

A technology of cyanobacteria and salt tolerance, which is applied in the field of salt tolerance SyDBSP derived from cyanobacteria and its application, can solve problems such as not achieving remarkable results, achieve high application possibility, increase salt tolerance, and salt tolerance sex increasing effect

Inactive Publication Date: 2013-07-10
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of farmland salinization, many scientists have tried to develop salt-tolerant crops through variety improvement methods such as hybridization, but no significant results have been achieved

Method used

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  • Salt tolerance SyDBSP gene derived from synechocystis, and uses thereof
  • Salt tolerance SyDBSP gene derived from synechocystis, and uses thereof
  • Salt tolerance SyDBSP gene derived from synechocystis, and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1: SyDBSP gene derived from Synechocystis sp. PCC6906

[0096] The base sequence of the SyDBSP gene derived from Synechocystis PCC6906 was isolated and determined after the genome of Synechocystis PCC6906 was isolated and all base sequence information was obtained using GS-FLX (Roche, USA). The SyDBSP gene consists of 471 nucleotides, encoding 156 amino acid sequences ( figure 1 and 2 ). The amino acid sequence of the SyDBSP gene derived from Synechocystis PCC6906 and Synechocystis PCC6803slr1894 produced in freshwater reached 80% (identity) and 91% positive (positivive), and its genetic relationship was the closest. In addition, it also showed a high genetic relationship with Synechocystis PCC7002A0031 and Cyanothece sp.PCC88014066 genes ( image 3 ).

Embodiment 2

[0097] Example 2: Screening of SyDBSP Gene Transformation Vectors and Transformed Plants Derived from Synechocystis PCC6906

[0098] In order to obtain transformed plants, from PCC6906 genomic DNA, use primers 5'-gctctagaATGACTTCAATTAATATCGGTATT-3' (SEQ ID NO.3, the Xba I site is underlined) and 5'-cgggatccCTATTTGTTCAGAACCCGGAGCAT-3' (SEQ ID NO. 4. The BamHI site is underlined) After the SyDBSP gene is amplified, it is cut with restriction enzymes. The XbaI / BamHI site of the pHC21B vector was cut with restriction enzymes, and the SyDBSP gene fragment was inserted thereinto, thereby preparing a nuclear transformation vector ( Figure 4 ). The plants introduced with the transformation vector are screened in a medium containing kanamycin as a selection marker. The selected plants use the primers of the SyDBSP gene to confirm the insertion of the SyDBSP gene by the PCR method. And, for the expression of SyDB SP gene in each transformed plant body, its expression degree was anal...

Embodiment 3

[0099] Example 3: Preparation of tobacco transformed with SyDBSP gene derived from Synechocystis sp. PCC6906

[0100] Retain and sterilize T0 generation seeds from tobacco transformed plants introduced with the SyDBSP gene derived from Synechocystis sp. , thus preserving the T1 generation seeds. Plants were obtained from the retained seeds, and genomic DNA was isolated, and the introduction of SyDBSP was confirmed by PCR and Southern blot hybridization analysis, and total RNA was isolated, and the expression level of the introduced genes was confirmed by real-time quantitative PCR. Such as Figure 5 As shown in A, among the 8 kinds of SyDBSP gene-transformed tobacco plants, 6 kinds of genes have been confirmed to have been introduced, and 5 kinds of these transformed plants have been confirmed to have inserted a copy of the gene ( Figure 5 B). In addition, in transformed tobacco plants, the overexpression of the SyDBSP gene can be confirmed by the results of real-time quan...

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Abstract

The present invention relates to a method for increasing the salt tolerance of a plant, to a plant having increased salt tolerance prepared by the method, and to a seed of the plant, wherein the method comprises over-expressing SyDBSP by transforming a plant cell with a recombinant plant expression vector including a gene encoding Synechocystis PCC 6906-derived SyDBSP protein and a SyDBSP gene.

Description

technical field [0001] The present invention relates to a Synechocystis putative DNA binding stress protein (SyDBSP) gene derived from cyanobacteria and a method for overexpressing the gene in plants, thereby increasing the salt tolerance of plants , and the plant body and its seeds with increased salt tolerance prepared by the method. Background technique [0002] Algae containing cyanobacteria have been exploited as efficient genetic resources, bioenergy (red algae ethanol and seaweed biodiesel) and efficient biomaterials (red algae slurry). Recently, the applicability of seaweed has been broadened through the application of seaweed bioengineering, and research on seaweed is underway. For example, new product development using molecular breeding, bioreactors for the production of effective proteins or effective substances that are important for medicine or industry. [0003] Recently, it has been reported that many genes found in cyanobacteria have been successfully appl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C07K14/195C12N15/31C12N15/82
CPCC07K14/195C12N15/8273C07K14/405C12Q1/6895Y02A40/146
Inventor 刘长烈金锡源金宗炫闵成兰郑元重安明淑朴英敏吴明珍朴祉泫
Owner KOREA RES INST OF BIOSCI & BIOTECH
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