Method for extracting cordyceps polysaccharide and adenosine from cordyceps sinensis mycelium
A technology for Cordyceps polysaccharide and Cordyceps sinensis, which is applied in the field of bioengineering, can solve the problems of complex separation process, low overall yield and small separation capacity, and achieves the effects of simplified process, short separation time and reduced cost.
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Embodiment 1
[0023] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, and ultrasonically treat for 15min at 33kHZ in an ice bath, then extract in a water bath at 75°C for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), Add 0.6g of potassium carbonate, then add 0.4g of ethanol, mix, stand still, and separate phases to obtain adenosine that is mainly distributed in the upper phase with a distribution coefficient of 29.5 and a recovery rate of 93.7%; polysaccharides are mainly distributed in the lower phase with a distribution coefficient of 0.07, the recovery rate was 85.6%.
Embodiment 2
[0025] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, then in 33kHZ, ice bath condition, ultrasonic treatment for 15min, 75℃ water bath extraction for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), add Add 0.3g of sodium carbonate, then add 0.4g of ethanol, mix, stand still, and separate the phases. The adenosine is mainly distributed in the upper phase, with a distribution coefficient of 49.4, and the recovery rate is 98.3%; the polysaccharide is mainly distributed in the lower phase, and the distribution coefficients are respectively 0.16, the recoveries were 68.3%.
Embodiment 3
[0027] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, then in 33kHZ, ice bath condition, ultrasonic treatment for 15min, 75℃ water bath extraction for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), add 0.4g of ammonium sulfate, then add 0.4g of ethanol, mix, stand still, and separate phases. Adenosine was mainly distributed in the upper phase with a distribution coefficient of 8.89 and a recovery rate of 84.9%; polysaccharides were mainly distributed in the lower phase with a distribution coefficient of 0.23 and a recovery rate of 87.3%.
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