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Polypeptide for inhibiting activity of bacillus subtilis transglutaminase (BTG) and its screening method and use

A technology of transglutaminase and Bacillus subtilis, which is applied in the biological field, can solve the problems of toxicity, low enzyme yield, and no patent publications have been found, and achieve the effects of high-efficiency expression, release of inhibitory effects, and mitigation of toxic effects

Active Publication Date: 2013-04-24
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because TG has protein cross-linking activity and has toxic effects on host cells, although Portuguese Placido et al. realized the expression of BTG in E. coli in 2007, the enzyme yield was very low under different induction and cu

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  • Polypeptide for inhibiting activity of bacillus subtilis transglutaminase (BTG) and its screening method and use
  • Polypeptide for inhibiting activity of bacillus subtilis transglutaminase (BTG) and its screening method and use
  • Polypeptide for inhibiting activity of bacillus subtilis transglutaminase (BTG) and its screening method and use

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Embodiment Construction

[0029] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0030] The methods in the following examples are conventional methods unless otherwise specified.

[0031] TG derived from Streptomyces has a signal peptide and a propeptide. Due to the action of the propeptide, TG derived from Streptomyces is inactive in cells and cannot function as a cross-linking protein, thereby not damaging cells. However, BTG derived from Bacillus subtilis has no signal peptide and propeptide, and only has an active mature peptide. Therefore, it is hoped that the activity of BTG can be inhibited by the propeptide of Streptomyces TG.

[0032] Selected and compared the TG propeptide sequences from 7 different Streptomyces (S.cinnamoneus, S.caniferus, S.fradiae, S.hygroscopicus, S.mobaraense, S.netropsis, S.platensis), re...

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Abstract

The invention relates to a polypeptide for inhibiting an activity of bacillus subtilis transglutaminase (BTG) and its screening method and use. The polypeptide has an amino acid sequence shown in the formula of SEQ ID No: 1. The polypeptide provided by the invention can inhibit the activity of BTG by a fusion protein and has an inhibition rate more than 60%. Through the polypeptide, the activity of expressed BTG having strong cytotoxicity is inhibited in a host cell and then is recovered after the inhibitor is removed under the action of a blood coagulation factor Xa.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a polypeptide for inhibiting the activity of bacillus subtilis transglutaminase (abbreviated as BTG for bacillus subtilis transglutaminase) and its screening method and application. Background technique [0002] Transglutaminase (EC2.3.2.13, TG for short) is an enzyme that catalyzes the acyl transfer reaction and can catalyze the formation of ε-(γ-Glu )-Lys isopeptide bonds make it covalently cross-linked and polymerized, thereby directly changing the structure and function of the protein itself and the cells and tissues to which the protein is attached. Therefore, the enzyme has a wide range of applications in the food industry and the pharmaceutical industry. TG production methods include animal and plant tissue extraction and microbial fermentation. The early production of TG used animal tissue extraction, but due to the lack of animal tissue sources, complex extraction process, ...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/70G01N21/64C12R1/54C12R1/55C12R1/585C12R1/465
Inventor 路福平刘逸寒蔺松王春霞王建玲
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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