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Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit

A chemiluminescence immunoassay and angiotensin technology, which is applied in the field of immunoassay medicine, can solve the problems of short validity period of reagents, narrow detection range, and low sensitivity, and achieve the effects of good stability, easy operation, and high sensitivity

Active Publication Date: 2015-05-13
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to provide Ang I magnetic particle chemiluminescence immunoquantitative detection kit and its preparation method, which avoids the shortcomings of radioimmunoassay reagents such as short validity period, radioactive pollution, and cumbersome operation, and solves the problem of low sensitivity, Detect narrow, costly defects

Method used

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  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit
  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit
  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of Angiotensin Ⅰ (Ang Ⅰ) Magnetic Particle Chemiluminescent Immunoquantitative Assay Kit Ⅰ

[0032] (1) Preparation of Ang Ⅰ calibrator:

[0033] Dilute the Ang Ⅰ pure product with 50% bovine serum to form a series of gradients, the concentrations are 0, 0.8, 2, 10, 20, 50ng / mL;

[0034] (2) Preparation of Ang Ⅰ quality control substance:

[0035] The Ang I antigen was prepared with 50% bovine serum for low-value quality control and high-value quality control, and the concentrations were 4.16ng / mL and 17.4ng / mL;

[0036] (3) Preparation of magnetic particle-streptavidin suspension:

[0037]Take 100mL0.1mol / L 2-morpholineethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in turn, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after reacting for 1h, use a magnetic stand to absorb, stand still for 10min, remove the liquid, add 10mL 0....

Embodiment 2

[0059] Example 2: Preparation of Angiotensin Ⅰ (Ang Ⅰ) Magnetic Particle Chemiluminescent Immunoquantitative Assay Kit Ⅱ

[0060] (1) Preparation of Ang Ⅰ calibrator:

[0061] Dilute the Ang Ⅰ pure product with 50% bovine serum to form a series of gradients, the concentrations are 0, 0.8, 2, 10, 20, 50ng / mL;

[0062] (2) Preparation of Ang Ⅰ quality control substance:

[0063] The Ang I antigen was prepared with 50% bovine serum for low-value quality control and high-value quality control, and the concentrations were 6.24ng / mL and 11.6ng / mL;

[0064] (3) Preparation of magnetic particle-streptavidin suspension:

[0065] Take 100mL0.1mol / L 2-morpholineethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in turn, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after reacting for 1h, use a magnetic stand to absorb, stand still for 10min, remove the liquid, add 10mL 0...

Embodiment 3

[0076] Example 3: Preparation of Angiotensin Ⅰ (Ang Ⅰ) Magnetic Particle Chemiluminescent Immunoquantitative Assay Kit III

[0077] (1) Preparation of Ang Ⅰ calibrator:

[0078] Dilute the Ang Ⅰ pure product with 50% bovine serum to form a series of gradients, the concentrations are 0, 0.8, 2, 10, 20, 50ng / mL;

[0079] (2) Preparation of Ang Ⅰ quality control substance:

[0080] Use Ang Ⅰ antigen containing 50% bovine serum to prepare low-value quality control and high-value quality control, the concentrations of which are 5.2ng / mL and 13.8ng / mL;

[0081] (3) Preparation of magnetic particle-streptavidin suspension:

[0082] Take 100mL0.1mol / L 2-morpholineethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in turn, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after reacting for 1h, use a magnetic stand to absorb, stand still for 10min, remove the liquid, add 1...

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Abstract

The invention discloses a quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I. The kit comprises Ang I calibrators, magnetic particle suspension coupled with streptavidin, an Ang I antibody labeled with biotin, an Ang I enzyme combination, an Ang I quality controller, chemiluminescence liquor A, chemiluminescence liquor B, 20 times concentrated washing liquor, and a reaction tube, wherein enzyme adopted by the Ang I enzyme combination is horse radish peroxidase with the purity RZ being more than or equal to 3.0 and the activity being more than or equal to 250U / ml. The invention also discloses a preparation method of the kit. Compared with the conventional kit, the quantitative detection kit is simple and convenient to operate, is safe, does not cause environment pollution, and also has the advantages of wide concentration range, low cost, good stability and the like of detection samples.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine. Specifically, the invention provides an angiotensin I (AngI) magnetic particle chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Angiotensin Ⅰ is converted from renin acting on angiotensinogen, so it can reflect the activity of renin. The renin-angiotensin-aldosterone system plays an important role in maintaining blood pressure, water and electrolyte balance, and internal environment stability. [0003] Angiotensin Ⅰ can stimulate the adrenal medulla to secrete adrenaline, and its effect of directly constricting blood vessels is not obvious; it can promote the adrenal cortex to secrete aldosterone, which acts on the renal tubules to retain sodium, water, and excrete potassium, thereby causing blood volume to increase Clinically, angiotensin Ⅰ is of great significance to the diagnosis and classification of hypertension, as well...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/531G01N33/532G01N21/76
Inventor 刘萍张影宋启超范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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