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Method for improving pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying myrosinase preparation in field

A mustard enzyme and preparation technology, applied in the field of comprehensive utilization of agricultural waste

Active Publication Date: 2013-03-20
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On March 15, 2012, the applicant submitted to the State Intellectual Property Office an invention patent application named Myrosinase-producing Trichoderma aureus and its application. The application number is 201210068201.6. Enzymes, at present, there is no report at home and abroad on the application of myrosinase produced by Trichoderma dark viridans in the field to improve the antibacterial and disease-preventing functions of rapeseed organic fertilizers

Method used

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  • Method for improving pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying myrosinase preparation in field
  • Method for improving pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying myrosinase preparation in field
  • Method for improving pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying myrosinase preparation in field

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The dark green Trichoderma used in this embodiment is the dark green Trichoderma registered and preserved by the applicant in the China Microbial Culture Collection Center, and its preservation number is: CGMCC NO.5609. This dark green Trichoderma has passed the Chinese invention of 201210068201.6 Patent applications are open to the public.

[0027] Preparation of Trichoderma dark viride culture medium

[0028] Preparation of test tube slant medium: 200 grams of potatoes, 20 grams of glucose or sucrose, 15-20 grams of agar, 1000 ml of water, natural pH. Peel the potatoes, cut into pieces and boil for half an hour, then filter with 2 layers of gauze, add sugar and agar, heat and boil until the agar melts, make up the volume to 1000 ml, cool it to about 60°C and put it into test tubes, the filling volume is 1 / 5-1 / 6 of the height of the test tube, plug the test tube mouth with a test tube stopper, autoclave at 121°C for 25 minutes, take it out while it is hot, and place i...

Embodiment 2

[0031] Preparation of myrosinase preparation

[0032] In the embodiment 1, Trichoderma dark viridifera preservation bacterial classification is inoculated into the test tube slant medium to activate the test tube slant surface, then, the activated bacterial classification is inoculated in the 250ml Erlenmeyer flask that 80ml liquid seed medium is housed with the inoculation loop, Put the triangular flask on a shaker for 24 hours, the speed of the shaker is 160r / min, and the culture temperature is 28±2°C; after the end, insert 2000ml of 800ml liquid production medium with 5% inoculum volume (V / V) In the triangular flask, place the triangular flask on a shaker for cultivation, the rotation speed of the shaker is 180r / min, and the cultivation temperature is 28±2°C. After 3-4 days of cultivation, the cultivation can be ended when the liquid culture starts to turn yellow; the liquid culture The material was filtered through four layers of gauze, and the hyphae on the gauze were col...

Embodiment 3

[0034] Growth Inhibitory Effects of Rapeseed Meal on Mycelia and Spores or Sclerotia of Phytopathogenic Fungi

[0035] All pathogenic fungi participating in the experiment can be obtained from the Microbial Culture Collection Center of the Chinese Academy of Sciences. All the pathogenic fungi participating in the test were inoculated into PDA plates, cultured at 28-30°C for 5 days, and punched with a hole punch to obtain mycelium blocks with a diameter of 4 mm for use.

[0036] Inhibitory effect of volatile decomposition products in rapeseed meal on the growth of phytopathogenic fungal mycelium: Pour PDA medium into a petri dish (diameter 8.5cm, height 1.3cm), and inoculate a 5 mm diameter fungus dish when it is placed at room temperature; Add 0.2g, 0.4g, 0.6g, 0.8g, 1.0g, 1.2g of rapeseed meal powder passed through a 2mm sieve to the center of the petri dish lid, add sterilized water at a ratio of rapeseed meal: water = 1:5 and mix thoroughly. Only add 6 mL of sterilized wat...

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Abstract

The invention relates to a method for improving the pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying a myrosinase preparation in the field. Glucosinolates in the rapeseed meal is rapidly degraded by adopting the myrosinase preparation for releasing butyl isosulfocyanate and further develop the pathogen-inhibiting and disease-controlling pesticide effect. The method is characterized by comprising the steps of: removing residue generated by previous crops in the field at a soil temperature of above 15 DEG C, carrying out rotary tillage for 20-25cm after a base fertilizer is applied to soil, uniformly spreading the rapeseed meal into the field, wherein the consumption of the rapeseed meal is 200-400kg / mu; uniformly spreading the myrosinase preparation in the field, wherein the consumption of the myrosinase preparation is 1-1.5kg / mu; carrying out rotary tillage and watering on the soil to ensure that the content of the water in the soil is 60-70 percent of the maximum water-holding capacity in the field, covering a plastic film, and coating for 15-20 days at a soil temperature of higher than 25 DEG C, coating for 20-30 days at a soil temperature of lower than 25 DEG C, and then removing the film for ventilating for 2-3 days so that the pathogen-inhibiting and disease-controlling pesticide effect of the rapeseed meal is improved.

Description

Technical field [0001] The present invention involves a method of applying mustard enzymes in the field to improve the effects of bacteriostatic disease and prevention and prevention.It belongs to the field of comprehensive utilization of agricultural waste. Background technique [0002] Rape is the third largest oil crop in the world. The production of rapeseed is second only to soybean and cottonseed. It is one of the most important oil crops.Since 1978, my country's rapeseed area and output have stabilized the world's first place, reaching 7 million hectares, accounting for 26%of the world's rapeseed area, which can produce about 10 million tons of cauliflower meal.The protein content in the cauliflower meal is as high as 40-45%, which is an excellent protein raw material. It has the potential for the development of the development of protein, but its adding ratio generally cannot be higher than 15-20%, because the vegetables contained in the cauliflower meal containedFor meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00A01B79/00C12N9/24C12R1/885
Inventor 马艳常志州杨豪张建英徐跃定
Owner JIANGSU ACAD OF AGRI SCI
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