Medium for mold quantity examination and preparation and application thereof
A culture medium and agar medium technology, applied in the field of biological analysis, can solve the problems affecting the accuracy, etc., and achieve the effect of comprehensive mold phase and accurate mold quantity results
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Embodiment 1
[0038] Prepare medium according to the following formula:
[0039] Potatoes 200g
[0040] Glucose 20.0g
[0041] NaCl30.0g
[0042] Agar 16.0g
[0043] Chloramphenicol 0.1g
[0044] Make up 1000mL with distilled water. Medium preparation method: Peel and cut potatoes into pieces, add 1000mL distilled water, and boil for 10min. Filter with gauze, add glucose, NaCl and agar, add distilled water to 1000mL, heat to dissolve, and then sterilize at 121°C for 20min. Before pouring the plate, dissolve chloramphenicol with a small amount of ethanol and add it to the medium.
Embodiment 2
[0046] Prepare medium according to the following formula:
[0047] Potatoes 300g
[0048] Glucose 15.0g
[0049] NaCl25.0g
[0050] Agar 25.0g
[0051] Make up 1000mL with distilled water.
[0052] Medium preparation method: Peel and cut potatoes into pieces, add 1000mL distilled water, and boil for 10min. Filter with gauze, add glucose, NaCl and agar, add distilled water to 1000mL, heat to melt, after aliquoting, sterilize at 121°C for 20min, pour plate.
Embodiment 3
[0054] Prepare medium according to the following formula:
[0055] Potatoes 200g
[0056] Glucose 25.0g
[0057] NaCl35.0g
[0058] Agar 15.0g
[0059] Chloramphenicol 0.1g
[0060] Make up 1000mL with distilled water.
[0061] The medium preparation method is the same as in Example 1.
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