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Capillary electrophoresis fluorescence detection device

A capillary electrophoresis and fluorescence detection technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of limited popularization and high price, and achieve the effect of reducing equipment cost, easy operation and high detection sensitivity

Inactive Publication Date: 2013-01-16
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: in order to overcome the shortcomings of the existing capillary electrophoresis fluorescence detection device which is expensive and restricts popularization and use, the present invention provides a capillary electrophoresis fluorescence detection device designed and constructed based on a microscope

Method used

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Experimental program
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Embodiment 1

[0025] image 3 Fluorescence spectrum and electrophoresis spectrum of quantum dots are detected by capillary electrophoresis fluorescence. The experimental conditions are: the length of the quartz capillary is 60cm, the inner diameter is 75μm; the buffer solution is 25mmol / L boric acid buffer solution, the pH value is 9.3; a 10kV high-voltage power supply is applied, and 10s electric sampling; after the electric sampling is completed, an 18kV separation Voltage; set the detection wavelength of the spectrometer to 612nm.

Embodiment 2

[0027] Figure 4 Electrophoretic spectrum of 5(6)-carboxyfluorescein detected by fluorescence capillary electrophoresis. The experimental conditions are the same as in Example 1, except that the detection wavelength of the spectrometer is 520nm.

Embodiment 3

[0029] Figure 5 For quantum dots and peptide molecules DDDLVPRGSGP 9 G 2 h 6 The electrophoretic spectrum of the conjugate. Figure 5 Curve a in the middle is the electrophoretic spectrum of quantum dots, and curve b is the quantum dots and polypeptide molecule DDDLVPRGSGP 9 G 2 h 6 Electrophoretic spectrum of the conjugate. The experimental conditions of this embodiment are the same as those of Embodiment 1, and the detection wavelength of the spectrometer is 612nm.

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Abstract

The invention relates to a capillary electrophoresis fluorescence detection device, comprising a high-voltage power source, a capillary tube, two buffer solution ponds, a fluorescence microscope, a fluorescence interface, a spectrograph and a data sampling and analyzing system. Two ends of the capillary tube are separately dipped in the two buffer solution ponds. The high-voltage power source has two electrodes, one end of which is separately connected with the anode and cathode of the high-voltage power source, and the other end of which is separately dipped in the two buffer solution ponds. The capillary tube is equipped in the focus of an objective lens and is fixed together with an objective table. One end of the fluorescence interface is connected with the standard interface of the fluorescence microscope, and the other end is connected with the spectrograph through an optical fiber, and furthermore, a compound lens is arranged in the fluorescence interface. The spectrograph is connected with the data sampling and analyzing system. The invention is easy to operate and high in the detection sensitivity, and can achieve electrophoretic separation and fluorescence detection. Design of the interface between the microscope and the spectrograph achieves the formation of complete fluorescence detection device by using microscopes, which greatly reduces cost of the equipment.

Description

technical field [0001] The invention relates to a capillary electrophoresis detection device, in particular to a capillary electrophoresis fluorescence detection device. Background technique [0002] Capillary electrophoresis (Capillary electrophoresis, CE) is a new electrophoresis method in which ions or charged particles are driven by an external electric field and separated in a capillary according to their mobility / partition coefficient differences. As a micro-separation analysis technology, CE combines the advantages of classic electrophoresis technology and modern micro-column separation technology, and has the characteristics of high resolution, high sensitivity, high speed, high throughput and low sample consumption (Galceran M.T., Diez M.J. Chromatogr. A, 1997, 782, 289-295). CE has been widely used in the field of bioanalysis in recent years because it meets the requirements for the separation and analysis of biomolecules in various fields of life sciences. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王建浩王车礼邱琳蒋鹏举
Owner CHANGZHOU UNIV
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