Method for in vitro flowering and pistil unisexual flowering of rose test-tube plantlets and culture medium thereof

A technology of test-tube seedlings and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of simultaneous flowering of diploid and haploid in test tubes, etc.

Inactive Publication Date: 2015-08-19
YANAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to current reports, whether it is tissue culture or flowering in test tubes, it is not the northern Shaanxi rose, and they have not achieved the level of diploid and haploid flowering at the same time in test tubes.

Method used

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Experimental program
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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific embodiments.

[0016] step one:

[0017] Cut the short stems of roses that were born in the current year to about 5 cm in length and bring them back to the laboratory. After rinsing under tap water for 5-10 minutes, use 15ml of 84 disinfectant and add distilled water to 100ml to soak for 6-8 minutes. Then transfer to the purification workbench for standby. In the purification workbench, sterilize rose stems with 75% ethanol + 1 drop of Tween 20 for 10-15 seconds, then transfer to 0.1% mercuric chloride + 1 drop of Tween 20 for 6 minutes to sterilize. Bacteria water wash 3 to 5 times. Cut off the poisoned part, and the remaining part contains at least one bud, and put it into the prepared MS medium.

[0018] After the liquid in the inserted stem section germinates and grows to a length of 3-5 cm, the newly formed buds are taken out and transferred to MS medium with 1.2 mg / L of 6-benzyl...

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PUM

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Abstract

The invention discloses a method for flowering test-tube plantlets of Chinese roses in a test tube and carrying out pistil monosexual flowering and a culture medium thereof. The method comprises the following steps: (1) obtaining rootless test-tube plantlets through subculturing; (2) flowering diploids of the rootless test-tube plantlets of Chinese roses in the test tube; and (3) carrying out induced-flowering on pistils of the diploids of the rootless test-tube plantlets of Chinese roses. According to the method, the flowering rate of diploids of test-tube plantlets can reach about 50%; and diploid flowers and pistil haploid flowers are flowered in a tower mode and exist for 20-35 days, and the flowering rate reaches about 30%.

Description

technical field [0001] The invention relates to a method for flowering in test tubes of rose test tube seedlings and unisexual flowering of pistils and a culture medium thereof, belonging to the field of plant biotechnology, plant genetic breeding or miniature flower production. Background technique [0002] Rose (R.chinensis), known as the queen of flowers, also known as "Moon Moon Red", belongs to the Rosaceae (Rosaceae) subfamily Rosaceae and belongs to the genus Rosa. Evergreen or semi-evergreen low shrubs, flowering in four seasons, mostly red, occasionally white, can be used as ornamental plants, medicinal plants, also known as roses. The natural flowering period is from May to November. The flowers are large and fragrant. They are widely used in horticultural cultivation and cut flowers. The types of rose flowers mainly include cut flower roses, edible roses, Fujimoto roses, and ground cover roses. China is one of the origins of rose. Wild roses in northern Shaanxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 齐向英陈宗礼张向前陈国梁薛皓孙志宏
Owner YANAN UNIV
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