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Method for culturing citric acid fermenting seeds and method for preparing citric acid by fermenting

A technology of fermentation medium and citric acid, which is applied in the field of citric acid fermentation seed culture and citric acid preparation by fermentation, can solve the problems of long fermentation period, long seed age and poor seed quality in the fermentation stage, so as to reduce the seed culture time and Fermentation cycle, mycelium and thickening, the effect of shortening the seed age

Active Publication Date: 2013-01-09
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new citric acid fermented seed culture in order to overcome the long seed age and poor seed quality of the seeds cultivated in the seed culture stage in the prior art, resulting in long fermentation period and low conversion rate in the fermentation stage. method and a method for fermenting and preparing citric acid

Method used

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  • Method for culturing citric acid fermenting seeds and method for preparing citric acid by fermenting
  • Method for culturing citric acid fermenting seeds and method for preparing citric acid by fermenting

Examples

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Embodiment 1

[0059] This example is used to illustrate the method for culturing citric acid fermented seeds and the method for fermenting and preparing citric acid provided by the present invention.

[0060] (1) The harvested corn is crushed to obtain a crushed product with an average particle diameter of 400 μm.

[0061] (2) Slurry the pulverized product at a concentration of 24% by weight, add 20 enzyme activity units of amylase (Novozymes, α-amylase, the same below) for each gram of the pulverized product, and enter the jet Device, under the conditions of 93°C and pH 5.9 for 100 minutes to obtain the product, namely the liquefied liquid.

[0062] (3) Part of the liquefied liquid is filtered by a hydraulic plate and frame filter press to separate the liquefied clear liquid and the enzymatic residue, wherein the solid content of the enzymatic residue is 51% by weight.

[0063] (4) Dilute part of the liquefied liquid in step (2) with water until the total sugar is 10% by weight to obtain ...

Embodiment 2

[0067] This example is used to illustrate the method for culturing citric acid fermented seeds and the method for fermenting and preparing citric acid provided by the present invention.

[0068] (1) The harvested corn was crushed to obtain a crushed product with an average particle diameter of 380 μm.

[0069] (2) Slurry the pulverized product at a concentration of 27% by weight, add 50 enzyme activity units of amylase per gram of the pulverized product, enter the injector, and enzymatically The product was obtained after solution for 110 minutes, that is, liquefied liquid.

[0070] (3) Part of the liquefied liquid is filtered by a hydraulic plate and frame filter press to separate the liquefied clear liquid and the enzymatic residue, wherein the solid content of the enzymatic residue is 50% by weight.

[0071] (4) Dilute part of the liquefied liquid in step (2) with water until the total sugar is 8% by weight to obtain a seed medium, put the seed medium into the seed tank, h...

Embodiment 3

[0075] This example is used to illustrate the method for culturing citric acid fermented seeds and the method for fermenting and preparing citric acid provided by the present invention.

[0076] (1) The harvested corn was crushed to obtain a crushed product with an average particle diameter of 370 μm.

[0077] (2) Slurry the pulverized product at a concentration of 26% by weight, add 15 enzyme activity units of amylase per gram of the pulverized product, enter the injector, and enzymatically The product was obtained after 120 minutes of solution, that is, the liquefied liquid.

[0078] (3) Part of the liquefied liquid is filtered by a hydraulic plate and frame filter press to separate the liquefied clear liquid and the enzymatic residue, wherein the solid content of the enzymatic residue is 49% by weight.

[0079] (4) Dilute part of the liquefied liquid in step (2) with water until the total sugar is 12% by weight to obtain a seed medium, put the seed medium into a seed tank,...

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Abstract

The invention discloses a method for culturing citric acid fermenting seeds. The method includes inoculating citric acid fermenting strains into a seed culture medium of a seed tank for seed culturing to obtain seed liquid. The method for culturing citric acid fermenting seeds is characterized in that pressure of the seed tank, ventilatory capacity and stirring rotation speed are all higher than previous pressure, ventilatory capacity and stirring rotation speed after seed culturing for 10-15 hours. The invention further discloses a method for preparing citric acid by fermenting. The method for preparing citric acid by fermenting includes subjecting the citric acid fermenting strains to seed culturing under the condition of seed culturing, inoculating the seed solution obtained after seed culturing into the fermenting culturing medium to ferment under the condition of generating citric acid so as to obtain fermenting liquid. The method for preparing citric acid by fermenting is characterized in that the citric acid is subjected to seed culturing according to the above method. By the methods, seed age can be shortened, seed quality is improved, fermenting period is shortened, conversion rate and final citric acid content are increased, consumption of power such as water and electricity is reduced and cost is saved.

Description

technical field [0001] The invention relates to the technical field of citric acid fermentation, in particular to a method for culturing citric acid fermentation seeds and a method for preparing citric acid by fermentation. Background technique [0002] Citric acid is one of the most used organic acids in the world today. So far, the citric acid consumed in the world is mainly produced by the fermentation method with Aspergillus niger as the strain. [0003] Citric acid fermentation production generally includes a seed tank culture stage and a fermenter fermentation stage. In the existing citric acid fermentation production, it is often only focused on the regulation of the fermentation stage of the fermenter, and the control of the seed tank cultivation stage is relatively extensive. Generally, the seed cultivation is only carried out under the conditions of fixed tank pressure, ventilation and stirring speed, for example The cultivation conditions include: temperature 30-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/48C12R1/685
Inventor 罗虎卢宗梅刘梦涵钟华章辉平
Owner COFCO BIOTECHNOLOGY CO LTD
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