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Artificially synthesized waterlogging-resistant gene sSub1A of maize and application thereof

A technology of artificial synthesis and corn, which is applied in the field of genetic engineering, can solve the problems of poor flood tolerance, unstable expression products, and low expression of transgenic corn, and achieve the effect of increasing yield and improving waterlogging tolerance

Active Publication Date: 2013-12-04
ANHUI QUANYIN SEED IND TECH
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Problems solved by technology

[0005] because Sub1 The gene is derived from rice, and its direct application to transgenic corn has defects such as low expression level, unstable expression product, and poor flood tolerance. Sub1 It is very necessary to carry out genetic modification to provide a gene sequence that can be stably and efficiently expressed in maize and significantly improve the waterlogging tolerance of maize, but there are no related reports so far

Method used

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  • Artificially synthesized waterlogging-resistant gene sSub1A of maize and application thereof
  • Artificially synthesized waterlogging-resistant gene sSub1A of maize and application thereof
  • Artificially synthesized waterlogging-resistant gene sSub1A of maize and application thereof

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Embodiment Construction

[0024] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0025] 1. Materials

[0026] pMD18-T SimpleVector (see map figure 1 ), CPB carrier (see map figure 2 ) was purchased from Bao Bioengineering (Dalian) Co., Ltd.; DH5α Escherichia coli competent cells and EH105 competent Agrobacterium cells were purchased from Bao Bioengineering (Dalian) Co., Ltd.; the plant genomic DNA extraction kit was Kangwei Century New Plant Genomic DNA Extraction kit; Reverse Transcription System reverse transcription kit was purchased from Promega; herbicide basta was purchased from Sangon Bioengineering (Shanghai) Co., Ltd.

[0027] The formula of YEP liquid medium is: 10 g of beef extract, 10 g of yeast extract, 5 g of NaCl, pH 7.0, and 1.5% agar is added to the corresponding fixed medium; MS and 1 / 4MS medium were purchased from Shanghai Dingjie Biotechnology Co., Ltd. Technology Co., Ltd.

[0028] 2...

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Abstract

According to the invention, a Sub1 gene of rice is redesigned and reconstructed to provide an artificially synthesized gene sequence for improving maize waterlogging tolerance. The gene sequence is one selected from the following: a ) a nucleotide sequence formed by a sequence shown as SEQIDNO.8; or b) a nucleotide sequence complementary to the nucleotide sequence in a). The invention also provides application of the gene sequence in improving waterlogging tolerance of plant, and recombinant vector containing the gene sequence and an engineered host cell. Experiments prove that the gene sequence provided by the invention can express stably and efficiently in maize and significantly improve waterlogging tolerance of maize, and has wide application prospects in cultivation of novel waterlogging-resistant maize and increase of maize yield.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to an artificially synthesized corn flood tolerance gene sSub1A and its application. Background technique [0002] Corn (zea mays L. ) is one of the most widely distributed food crops in the world, and it is also an important economic crop. In my country, its distribution range and planting area are very large, and it is one of the main grains of the people in the dry valley areas of our country. However, every year due to floods, maize yields suffer huge losses. At present, conventional maize breeding lacks flood-tolerant germplasm resources, and the breeding process is relatively slow. It is important to introduce flood-resistant genes into maize through modern biotechnology to cultivate new maize varieties with flood resistance. measure. [0003] Flooding stress creates a hypoxic environment, and maize is induced in an anoxic environment ADH Changes in gene expr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29
Inventor 朱苏文姜威姜翠萍周健江海洋
Owner ANHUI QUANYIN SEED IND TECH
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