Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus thuringiensis, as well as preparation method and application thereof

A technology of Bacillus thuringiensis and Bacillus thuringiensis, which is applied in botany equipment and methods, biochemical equipment and methods, applications, etc., and can solve the problems of Bacillus thuringiensis spores and crystals being small, easily affected by environmental factors, and easy to degrade active ingredients , to achieve unique insecticidal properties, mature industrial production technology, and reduce control costs

Active Publication Date: 2013-05-01
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production and application of Bacillus thuringiensis insecticides still have shortcomings such as narrow insecticidal range, easy degradation of active ingredients, and susceptibility to environmental factors.
And in production practice, due to artificial continuous subculture, Bacillus thuringiensis spores and crystals become smaller, growth rate slows down, insecticidal effect is low and other shortcomings, so it is still necessary to directly isolate more efficient and broad-spectrum Bacillus thuringiensis directly from nature for the development of insecticides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus thuringiensis, as well as preparation method and application thereof
  • Bacillus thuringiensis, as well as preparation method and application thereof
  • Bacillus thuringiensis, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of bacillus thuringiensis, the steps are:

[0032] A. Utilize the characteristic that the spores of Bacillus thuringiensis can withstand high temperature of 78-82 ℃, adopt the spring soil method: 5g of soil (Changsha, Hunan) is dried and ground into powder, sprinkled on sterilized filter paper with a size of 8 × 8cm, and make the surface of the filter paper Evenly stick a layer of sample powder on the surface, and flick the side with the sample against the petri dish for 1-2 times, and pour 80°C nutrient agar into the petri dish. Cultivate at 30°C for 5-7 days (Acta Microbiologica Sinica 30, 380-388), and observe the single colonies formed by microscopy to isolate Bacillus thuringiensis.

[0033] B. Using the newly hatched beet armyworm and cotton bollworm as test insects, the virulence bioassay was carried out with reference to GB / T19567.2-2004, and the highly virulent strain BtCS36 was screened after comparing the virulent lethal medium LC50.

[...

Embodiment 2

[0042] A kind of application of bacillus thuringiensis in the preparation of insecticide fermented liquid, its steps are:

[0043] A. Shake flask fermentation

[0044] The purpose of shake flask fermentation is to select the best fermentation formula, optimize the fermentation conditions, and conduct orthogonal experiments with different solid contents and different C / N ratios. Scraped cultures from plates cultured for more than 3 days were dissolved in sterile water to make spore suspension, treated at 75°C for 20 minutes for inoculation. 20 / 250ml volume, 30°C, 250rpm shake flask culture. The optimal medium formula of BtCS36 obtained is shown in Table 2.

[0045] B. A small test in a fully automatic fermenter

[0046] Using the best fermentation formula screened out, more than 10 fermentation tests were carried out in the fully automatic fermentation tank to determine the best fermentation process: the pH of the medium before elimination was 8.1, and the pH after eliminati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacillus thuringiensis, as well as a preparation method and an application thereof. The collection number of the bacillus thuringiensis is CCTCC M2012040. The method comprises the following steps: A, according to the characteristic that a spore of the bacillus thuringiensis can resist 78 to 82 DEG C high temperature and can form a parasporal crystal, the bacillus thuringiensis is separated according to an earth bomb method; and B, a just hatched beet armyworm or a cotton bollworm is adopted as a test worm for virulence bioassay, a middle quantity of LC50 is killed through virulence comparison, and a high-virulence strain BtCS36 is obtained by screening. The formula of a fermentation culture medium and a fermentation process are defined by utilizing an orthogonal experiment and small scale fermentation. The fermentation period is about 30 h by utilizing the bacillus thuringiensis and the defined fermentation process; and the virulence of the fermentation liquid works on cotton bollworms with 3000 to 5000IU / Mul. The production of the novel Bt pesticide not only can efficiently control various lepidoptera pests and ensure high and stable yield of crops, but also has important practical significance and urgency on the safety of the ecological environment.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to a bacillus thuringiensis, a preparation method of the bacillus thuringiensis, and an application of the bacillus thuringiensis in the preparation of an insecticide fermentation liquid. Background technique [0002] In order to expand the insecticidal spectrum and improve the insecticidal activity of Bacillus thuringiensis, at present both at home and abroad are committed to the isolation and identification of new strains and the development of new insecticidal genes. In terms of isolation, identification and screening of Bacillus thuringiensis strains, the literature "Screening and biological activity determination of Bacillus thuringiensis in Sanya area (Anhui Agricultural Science, 2009, 37(28): 13660-13661)", "Bacillus thuringiensis in Damaoshan area Research on distribution and diversity (China Agricultural Science Bulletin, 2011, 27(01): 166-169), "Isolation and i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04A01N63/02A01P7/04C12P1/04C12R1/07
Inventor 高梅影刘朋明吴燕吴丹丹
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com