Method for improving vitality and cold resistance of corn seeds
A technology for seeds and corn is applied in the field of improving the vigor and cold resistance of corn seeds, which can solve the problems of easy reduction of vigor and production loss, and achieve the effects of large processing capacity, simple operation and simple and easy seed soaking method.
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Embodiment 1
[0018] Example 1: Take 30 sweet corn seeds (W22 sweet corn reporter line) with full grain size and similar size, soak them in 70% ethanol for 5 minutes, soak them in 0.1% mercuric chloride solution for 8 minutes, and wash them with water. The seed soaking solution used was 5 μg / ml Hrf protein solution, and the surface spreading agent Silwet L-77 was added at a final concentration of 0.3%. The surface-sterilized corn seeds were placed in 5 μg / ml Hrf protein solution and soaked at 28° C. for 12 hours. Get the treated seeds and sow them in a conventional way.
Embodiment 2
[0020] Take 30 sweet corn seeds (W22 sweet corn reporter line) with full grain size and similar size, soak them in 70% ethanol for 5 minutes, soak them in 0.1% mercuric chloride solution for 8 minutes, and wash them with water. The seed soaking solution used was 10 μg / ml Hrf protein solution, and the surface spreading agent Sylgard 309 was added at a final concentration of 0.1%. The surface-sterilized corn seeds were placed in 5 μg / ml Hrf protein solution and soaked at 28° C. for 12 hours. Get the treated seeds and sow them in a conventional way.
Embodiment 3
[0022] Take 30 sweet corn seeds (W22 sweet corn reporter line) with full grain size and similar size, soak them in 70% ethanol for 5 minutes, soak them in 0.1% mercuric chloride solution for 8 minutes, and wash them with water. Put the surface-sterilized corn seeds in 10 μg / ml Hrf protein solution, add the surface spreading agent Silwet L-77 with a final concentration of 0.3%, and soak the seeds at 25°C for 24 hours. Get the treated seeds and sow them in a conventional way.
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