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Cell separation medium and cell separation method

A technology for separating medium and nuclear cells, which is applied in the field of compound cell separation medium and cell separation, can solve the problems of increasing cost, prolonging the production cycle, increasing the complexity of industrialized cell production, etc., and achieving low raw material uncertainty and high safety Effect

Active Publication Date: 2012-07-04
BEIJING JING MENG STEM CELL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The existence of the above potential risks and unknown factors lead to the strictest control of Ficoll 400 to prevent it from entering the human body
Since the separation medium will inevitably exist in the final cell product, there must be strict elution steps and residual detection to prove that Ficoll 400 is reduced to a sufficiently low level; these steps increase the cost, lengthen the production cycle, and improve industrialization. Complexity of Cell Production

Method used

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  • Cell separation medium and cell separation method
  • Cell separation medium and cell separation method
  • Cell separation medium and cell separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1, the preparation of formula A separation medium

[0062] In an environment with local 100-level laminar flow purification conditions, at 20°C, those skilled in the art who have been trained in aseptic techniques used conventional methods to respectively prepare Dextran 70, hydroxyethyl Starch (200 / 0.5) and a certain amount of sodium diatrizoate are fully dissolved in sterile water for injection to form a clear and transparent colorless or slightly yellow aqueous solution. The mass fractions of the three substances are 2.25%, 2.25%, and 9.0%, respectively. Then filter through a 0.22μm filter membrane into a sterile, endotoxin-free container.

[0063] At 20°C, the main characteristic parameters of the mixed aqueous solution include: (1) The density is 1.074g / cm 3 (See figure 1 , refer to method ); (2) osmotic pressure is physiological osmotic pressure (see figure 2 , between 285mOsm / kg and 310mOsm / kg) (refer to method ); (3) pH is between 6.0 and 9.0; (4)...

Embodiment 2

[0073] Embodiment 2, the preparation of formula B separation medium

[0074] In an environment with local 100-level laminar flow purification conditions, at 20°C, those skilled in the art who have been trained in aseptic techniques used conventional methods to respectively prepare Dextran 70, hydroxyl Ethyl starch (200 / 0.5) and a certain amount of sodium diatrizoate are fully dissolved in sterile water for injection to form a clear and transparent colorless or slightly yellow aqueous solution. The mass fractions of the three substances are 3.0%, 1.5%, and 9.0%, respectively. %, and then filtered through a 0.22μm filter membrane into a sterile, endotoxin-free container.

[0075] At 20°C, the main characteristic parameters of the mixed aqueous solution include: (1) The density is 1.074g / cm 3 (See figure 1 , refer to method ); (2) osmotic pressure is physiological osmotic pressure (see figure 2 , between 285mOsm / kg and 310mOsm / kg) (refer to method ); (3) pH between 6.0 and 9....

Embodiment 3

[0077] Embodiment 3, the preparation of formula C separation medium

[0078] In an environment with local 100-level laminar flow purification conditions, at 20°C, those skilled in the art who have been trained in aseptic techniques used conventional methods to respectively prepare Dextran 70, hydroxyl Ethyl starch (200 / 0.5) and a certain amount of sodium diatrizoate are fully dissolved in sterilized water for injection to form a clear and transparent colorless or slightly yellow product with mass fractions of 2.45%, 2.45%, and 9.0% respectively. The aqueous solution is then filtered through a 0.22μm filter membrane into a sterile, endotoxin-free container.

[0079] At 20°C, the main characteristic parameters of the mixed aqueous solution include: (1) The density is 1.075g / cm 3 (See figure 1 , refer to method ); (2) osmotic pressure is physiological osmotic pressure (see figure 2 , between 285mOsm / kg and 310mOsm / kg) (refer to method ); (3) pH between 6.0 and 9.0 (see imag...

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Abstract

The invention discloses a composition for separating a mononuclear cell and a compound lymphocyte separation medium. All raw materials in a formula of the compound lymphocyte separation medium respectively meet the standard of intravenous injection-grade bulk drugs and are high in safety; the purity of the recovered lymphocyte and the recovery rate of the lymphocyte have no remarkable difference from those of a conventional separation medium control group; and the lymphocyte obtained by separating respectively has equivalent effects to those of the control group in four indexes, namely multiplication, morphology, a surface marker and cytotoxicity activity, and is favorable in clinical application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a compound cell separation medium and a cell separation method. Background technique [0002] Cell therapy technologies such as immune cell therapy and stem cell therapy have greatly promoted the development of modern medical technology. In the process of cell therapy, the collection and isolation of target cells is an important step. The acquisition of high-purity target cells is the prerequisite for obtaining high-quality cell end products, namely effector cells. In the process of cell therapy, in addition to the need to use effector cells for treatment, there is also a type of auxiliary product used to obtain effector cells, namely cell therapy adjuvant materials, which are used for the isolation, growth, storage, transportation and infusion of effector cells And many other links, because they do not appear in the final product of cell therapy, their importance is usually ignore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0786C12N5/078
Inventor 赵侃樊晓翔高长青丁银巧高锦
Owner BEIJING JING MENG STEM CELL TECH
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