Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for quickly and quantificationally detecting lactobacillus plantarum ST-III

A Lactobacillus plantarum, quantitative detection technology, applied in biochemical equipment and methods, microbial determination/inspection, fluorescence/phosphorescence, etc., to achieve the effects of fast detection speed, shortened experimental period, and simple operation

Active Publication Date: 2013-07-17
BRIGHT DAIRY & FOOD CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a rapid, specific, high sensitivity, and low-cost method for quantitatively detecting Lactobacillus plantarum ST-III for the current situation that there is no molecular detection method for Lactobacillus plantarum ST-III strain. Method and its kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for quickly and quantificationally detecting lactobacillus plantarum ST-III
  • Method and kit for quickly and quantificationally detecting lactobacillus plantarum ST-III
  • Method and kit for quickly and quantificationally detecting lactobacillus plantarum ST-III

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Quantitative detection method of plantarum lactobacillus in embodiment 1 probiotic milk products

[0058] a. Preparation of sample total RNA

[0059] Extraction by Trizol method: The fermented milk drink containing Lactobacillus plantarum ST-III (Bright Changyou plant lactic acid bacteria drink) purchased on the market was used as the implementation sample, which were three samples that were 19 days, 15 days and 13 days away from the production date. The sample was ground in a mortar filled with liquid nitrogen, then quickly added 1mL Trizol reagent, according to the instructions of the Trizol kit ( Plus RNA Purification System, U.S. Invitrogen Company, Cat. No. 12183-555) extract sample RNA, then treat twice with DNaseI to ensure that the DNA contamination in the RNA is completely eliminated, obtain a purified RNA sample, measure the concentration, and pass through 1% agar for RNA integrity Sugar gel electrophoresis detection.

[0060] b. Reverse transcription ampli...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and a kit for quickly and quantificationally detecting lactobacillus plantarum ST-III. The method comprises the following steps of: 1) extracting total RNA (ribonucleic acid) of a sample to be detected; 2) carrying out inverse transcription on the total RNA extracted in the step 1) to obtain cDNA (complementary deoxyribonucleic acid); 3) carrying out fluorescent quantitative PCR (polymerase chain reaction) amplification detection by utilizing a primer pair of specifically amplified lactobacillus plantarum ST-III and taking the cDNA obtained in the step 2) as atemplate; and 4) comparing the circulation thresholds of the sample to be detected and a quantitative external standard product to quantify the actual number of copies in the sample to be detected. The method and the kit can quickly detect the quantity of lactobacillus plantarum ST-III in a detected sample and have fast speed, strong specificity and high sensitivity, and the limit of detection can be up to 2.85*10<3>CFU / mL.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a method for rapid quantitative detection of plantarum lactobacillus ST-III and a kit thereof. Background technique [0002] Probiotics are "living microorganisms that, when ingested in certain quantities, can have a beneficial effect on the health of the host". With the development of biotechnology and medicine and other disciplines, the beneficial effects of probiotics on human health have been confirmed more and more. Therefore, probiotic products are increasingly sought after by people, and their production and sales are also on the rise year by year. A large number of studies have shown that only the number of viable bacteria in the probiotics in the product reaches 1×10 6 Only one / ML can play its due effect. However, due to the lack of systematic research and formulation of standards for probiotic products in my country, especially the lack of strict restric...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/06G01N21/64
Inventor 陈臣任婧周方方艾连中
Owner BRIGHT DAIRY & FOOD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com