Method for producing microbial grease by fermenting inulin serving as raw material
A microbial oil and a technology using inulin, which is applied in the field of microbial fermentation to produce biological oil, can solve the problems of incomplete value development and energy shortage of Jerusalem artichoke tubers, achieve the effects of expanding the source of oil raw materials, reducing costs, and solving insufficient value utilization
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Embodiment 1
[0025] A kind of method that uses inulin as raw material fermentation to produce microbial oil comprises the following steps:
[0026] 1) Hydrolysis of inulin raw material
[0027] Dissolve inulin with a purity of 90% in distilled water at a ratio of 7%, add filter-sterilized inulinase at an enzyme dosage of 60 U / g, and heat-preserve and hydrolyze at 60°C for 12 hours to obtain an inulin hydrolyzate ,spare;
[0028] 2) Fermentation and cultivation of oily microorganisms
[0029] ①Cultivation of seed liquid: under aseptic conditions, pick two-ring strains from the activated fresh Trichosporium dermatosus slant and inoculate them into the sterilized bean sprout juice seed liquid medium, keep the temperature at 30°C, Under the condition of the shaking flask rotation speed of 150r / min, the shaking flask cultivation time was 72h, and the fermented seed liquid was obtained, which was set aside;
[0030] The components of the bean sprouts juice medium are (g / L): 100 bean sprouts, ...
Embodiment 2
[0037] A kind of method that uses inulin as raw material fermentation to produce microbial oil comprises the following steps:
[0038] 1) Hydrolysis of inulin raw material
[0039] Dissolve inulin with a purity of 90% in distilled water at a ratio of 7%, add filter-sterilized inulinase at a rate of 80 U / g, and heat-preserve and hydrolyze at 60°C for 10 hours to obtain inulin hydrolysis liquid, spare;
[0040] 2) Fermentation and cultivation of oily microorganisms
[0041] ①Cultivation of seed liquid: under aseptic conditions, pick two-ring strains from the activated fresh Trichosporium dermatosus slant and inoculate them into the sterilized bean sprout juice seed liquid medium, keep the temperature at 30°C, Under the condition of the shaking flask rotation speed of 150r / min, the shaking flask cultivation time was 72h, and the fermented seed liquid was obtained, which was set aside;
[0042] The components of the bean sprouts juice medium are (g / L): 100 bean sprouts, 50 sucr...
Embodiment 3
[0049] A kind of method that uses inulin as raw material fermentation to produce microbial oil comprises the following steps:
[0050] 1) Hydrolysis of inulin raw material
[0051] Dissolve inulin with a purity of 90% in distilled water at a ratio of 7%, add filter-sterilized inulinase at a rate of 100 U / g, and heat-preserve and hydrolyze at 60°C for 8 hours to obtain inulin hydrolysis liquid, spare;
[0052] 2) Fermentation and cultivation of oily microorganisms
[0053] ①Cultivation of seed liquid: under aseptic conditions, pick two-ring strains from the activated fresh Trichosporium dermatosus slant and inoculate them into the sterilized bean sprout juice seed liquid medium, keep the temperature at 30°C, Under the condition of the shaking flask rotation speed of 150r / min, the shaking flask cultivation time was 72h, and the fermented seed liquid was obtained, which was set aside;
[0054] The components of the bean sprouts juice medium are (g / L): 100 bean sprouts, 50 sucr...
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