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Method and device for detecting human blood types

A detection method and blood type technology, applied in the direction of material impedance, can solve the problems of limited application and popularization, high cost, and long detection time of trapezoidal microplate method, so as to ensure the safety of blood transfusion, easy storage, reduce detection cost and instrument manufacturing cost Effect

Active Publication Date: 2013-07-10
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The trapezoidal microplate method takes a long time to detect (generally takes 1 hour), while the card-type microcolumn gel method needs to purchase gel cards, which is very expensive (generally 50-80 yuan / case)
These shortcomings greatly limit the application of these two methods

Method used

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  • Method and device for detecting human blood types
  • Method and device for detecting human blood types
  • Method and device for detecting human blood types

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of standard antibody serum

[0048] Taking the preparation of standard anti-A serum as an example, the anti-A type serum was diluted with normal saline in a series of ratios to form 16 tubes of antibody serum with gradient concentrations. 40 uL of serum of each concentration was drawn and added to a 16-well Diana IgG cassette gel column, and then 30 uL of 2% type A Rh-positive red blood cell suspension was added to each well. After incubation for 15 min, centrifuge at 3000 rpm for 10 min. According to the standard of the Diana gel column detection method, six concentrations of antibody serum with agglutination strengths of -, ±, +, ++, +++, and ++++ were selected as standard antibody serum.

[0049] 2. Six types of red blood cell suspension sample preparation.

[0050] Take six test tubes, add 2 mL of 5% type A red blood cell suspension, and then add 0.3 mL of six kinds of standard antibody serum, shake and mix for 30 s, and let it stand for 2 minutes t...

Embodiment 2

[0054] 1. Preparation of standard antibody serum

[0055] Taking the preparation of standard anti-B serum as an example, the anti-B type serum was diluted with normal saline in a series of ratios to form 16 tubes of antibody serum with gradient concentrations. 40 uL of serum of each concentration was drawn and added to a 16-well Diana IgG cassette gel column, and then 30 uL of 2% type A Rh-positive red blood cell suspension was added to each well. After incubation for 15 min, centrifuge at 3000 rpm for 10 min. According to the standard of the Diana gel column detection method, six concentrations of antibody serum with agglutination strengths of -, ±, +, ++, +++, and ++++ were selected as standard antibody serum.

[0056] 2. Six types of red blood cell suspension sample preparation.

[0057] Take six test tubes, add 2 mL of 5% type B erythrocyte suspension, and then add 0.3 mL of six kinds of standard antibody serum, shake and mix for 30 s, and let it stand for 2 minutes to p...

Embodiment 3

[0061] 1. Preparation of standard antibody serum

[0062] Taking the preparation of standard anti-D serum as an example, the anti-D typing serum was serially diluted with normal saline to form 16 tubes of antibody serum with gradient concentrations. 40 uL of serum of each concentration was drawn and added to a 16-well Diana IgG cassette gel column, and then 30 uL of 2% type A Rh-positive red blood cell suspension was added to each well. After incubation for 15 min, centrifuge at 3000 rpm for 10 min. According to the standard of the Diana gel column detection method, six concentrations of antibody serum with agglutination strengths of -, ±, +, ++, +++, and ++++ were selected as standard antibody serum.

[0063] 2. Six types of red blood cell suspension sample preparation.

[0064] Take six test tubes, add 2 mL of 5% type D erythrocyte suspension, and then add 0.3 mL of six kinds of standard antibody serum, shake and mix for 30 s, and let it stand for 2 minutes to prepare the ...

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Abstract

The invention relates to a method and a device for detecting human blood types. The device comprises a program-controlled signal generation module, a data acquisition module, a data analysis module, a blood type identification module and a display and storage module, wherein the program-controlled signal generation module is used for controlling a program-controlled signal generator to excite a voltage signal; the data acquisition module is used for acquiring a return signal; the data analysis module is used for analyzing the signal; the blood type identification module is used for identifying the blood types; the display and storage module is used for displaying and storing the detected human blood types; an impedance detection device comprises a microcontroller, the program-controlled signal generator, an exciting electrode, a magnitude-phase detector, a detection electrode, a magnitude-stabilizing circuit, a constant-flow source circuit, a module conversion circuit and a signal conditioning circuit; and the program-controlled signal generator, the exciting electrode, the detection electrode, the magnitude-stabilizing circuit, the constant-flow source circuit, the module conversion circuit and the signal conditioning circuit are electrically connected. According to the method and the device disclosed by the invention, the detection cost and the manufacturing cost of an instrument can be reduced; the popularization of an automatic blood type equipped instrument towards medium-small sized hospitals and local blood stations is facilitated; the detection sensitivity is improved; the accuracy and the reliability of the qualification result are ensured; and the blood transfusion safety of people is ensured.

Description

technical field [0001] The invention relates to a detection method and device for human blood type, in particular to a detection method and device for detecting human ABO blood type by electrochemical means. Background technique [0002] ABO blood type is based on the presence or absence of A antigen and / or B antigen on the surface of the red blood cell membrane, blood types are divided into four types: A, B, AB, and O; Rh blood type is based on the presence or absence of D antigen on the surface of the red blood cell membrane and the strength of D antigen , the blood type is divided into Rh positive, Rh negative, weak D / incomplete D. The blood type of red blood cells can be detected by using known anti-A, anti-B and anti-D serum to determine whether there are corresponding A, B, and D antigens on red blood cells. When there is no certain antigen on the red blood cells, it will not react with the typed serum; however, when there is a certain antigen on the red blood cells, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/02
Inventor 廖强陈里里杨正书
Owner CHONGQING UNIV
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