Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof

A technology for peroxides and biological specimens, applied in the field of medical testing, can solve the problems of low specificity and sensitivity, low sensitivity, and short storage time of APF antigen sheets, and achieve the effect of low cost and good sensitivity

Active Publication Date: 2015-05-27
ARMY MEDICAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]In the early diagnostic indicators, there is a relationship between the increase of IL-8 and IL-6 levels in CMV DNA-positive rheumatoid arthritis patients and the appearance of CMV genome Linked, but low specificity and sensitivity
In terms of RA autoantibodies as detection indicators: Rheumatoid factor (RF) has the disadvantage of low sensitivity; 53.3% of patients with early RF-negative rheumatoid arthritis were positive for anti-perinuclear factor (APF), but APF antigen The storage time of tablets is short (only 1-2 weeks), and the detection stability is low; the positive rate of Sa antibody in rheumatoid arthritis is 40%, and the specificity is 98.9%, but only about 23% in the early stage of rheumatoid arthritis ; No one has reported the correlation between peroxide reductase Ⅳ and rheumatoid arthritis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof
  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof
  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The antigen-coated human peroxide reductase IV described in this example was obtained by constructing a prokaryotic expression plasmid, transforming the expression bacteria, and fermenting and culturing a large amount of bacterial precipitates containing peroxide reductase IV. Goat anti-human IgM labeled with root peroxidase (HRP) was used as the secondary antibody of the indirect method ELISA; the coating concentration of human peroxide reductase IV and the optimal concentration range of detection serum and the negative and positive critical value were further determined. To confirm, a specific and sensitive detection method for peroxide reductase Ⅳ antibody in rheumatoid arthritis was developed, which can capture 7.8 ng / mL peroxide reductase Ⅳ anti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided is an ELISA reagents kit for detecting peroxiredoxin IV antibody in biological samples, comprising an antibody capture agent, an enzyme-labeled secondary antibody and a substrate. Also provided are applications for the kit in detecting volumes of peroxiredoxin IV antibody and rheumatoid arthritis.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to an indirect ELISA kit for detecting peroxide reductase IV antibody in a biological sample and its method and application. Background technique [0002] Rheumatoid arthritis (RA) is a systemic autoimmune disease with synovitis as the basic pathological change and characterized by chronic destructive joint disease. Seriously disabled, seriously affecting the quality of life of patients. Rheumatoid arthritis without proper treatment can persist and even lead to joint deformities. Pathogenic antigen-driven autoreactive CD4 as an autoimmune disease + T cell activation is the core pathway in the pathogenesis of rheumatoid arthritis. [0003] For the diagnosis of rheumatoid arthritis, the mainstream still uses the 1987 American College of Rheumatology RA classification criteria, which mainly rely on clinical manifestations and exclude other arthritis to judge RA. In addition, X-ray ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCC07K16/40G01N33/573G01N33/577G01N33/6893G01N2800/102
Inventor 吴乔吴玉章
Owner ARMY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com