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Polymerase chain reaction (PCR) detection primer for goose-derived component

A goose-derived, one-to-one technology, applied in the field of identification of animal species and animal-derived components, can solve problems such as variation

Inactive Publication Date: 2012-02-01
SHENZHEN AUDAQUE DATA TECH
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  • Abstract
  • Description
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Problems solved by technology

(3) Although the mitochondrial genome sequences of different species of animals are highly homologous and have highly conserved regions, there are also certain regions of variation

Method used

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  • Polymerase chain reaction (PCR) detection primer for goose-derived component
  • Polymerase chain reaction (PCR) detection primer for goose-derived component
  • Polymerase chain reaction (PCR) detection primer for goose-derived component

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Embodiment Construction

[0018] The invention is described in further detail below with reference to the preferred embodiments shown. It should be understood that the examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.

[0019] 1. Design of primers: Design a pair of primers in the D-Loop region according to the goose mitochondrial genome sequence published in NCBI (accession number: EU571957), the sequence is as follows:

[0020] Upstream primer F: 5'-GTGGCTATTCCCAGTGAT-3';

[0021] Downstream primer R: 5'-TATTTGGAAGTGGTGGGT-3'.

[0022] 2. Extraction of total DNA from the sample

[0023] Use chloroform extraction or commercial DNA extraction kits to extract genomic DNA from geese, turkeys, ducks, quails, ostriches, pigeons, chickens, horses, cattle, sheep, pigs, donkeys, dogs, cats, and fish, and the extracted genomes The purity and concentration of DNA were determined by UV spectrophotometer. Determination of OD 260 / OD 280...

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Abstract

The invention relates to the technical field of identification of animal species and animal-derived components, in particular to a method for detecting a goose-derived component and a primer for detecting a nucleotide sequence of goose cell mitochondria, which aim to identify whether the goose-derived component is contained in products such as feeds, foods and the like or not. Due to the design of a specific amplification primer, mitochondrion deoxyribonucleic acid (DNA) which is independent of one another genetically, does not have repeated sequences and does not have tissue specificity in a biological individual is detected by using a polymerase chain reaction (PCR) technology, and a PCR detection method for the goose-derived component is established, so that the aim of quickly and accurately detecting and identifying the goose-derived component is fulfilled.

Description

technical field [0001] The invention relates to the technical field of identification of animal species and animal-derived components, in particular to a detection method for detecting goose-derived components, in particular to a pair of primers for detecting goose cell mitochondrial nucleotide sequences. Background technique [0002] The technology of identification and detection of livestock and poultry-derived components is a key qualitative technology in the entry-exit customs, trade, inspection and quarantine of meat food and feed, involving meat food processing industry, animal husbandry, feed industry, inspection and quarantine departments and related management departments, etc. field. However, there is no fast, accurate and reliable detection method for the species identification of goose and whether the product contains goose-derived components. [0003] The target fragment of the PCR amplification using the primers of the present invention is the mitochondrial DN...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 宗卉汪燕玲曹琛福陶虹刘建利吕建强花群义卢体康秦智锋
Owner SHENZHEN AUDAQUE DATA TECH
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