Prostate cancer methylation assay
A technology for prostate cancer and hypermethylation, applied in the field of prostate cancer methylation analysis, can solve the problems of large number of biopsies and low detection specificity
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[0007] Hypermethylation assays including detection of GSTP, APC, and RARβ2 markers are described, for example, in US Patent Publication 20080254455, which is hereby incorporated by reference. These assays have now been improved and can be combined with other diagnostic and risk factor indicators.
[0008] In a study of a cohort of 337 apparently healthy men with no previous history of prostate cancer, urine samples were obtained from 9 different urology clinical sites. Urine samples (up to 40 mL) were collected after the prescribed DRE, which involved indenting the prostate surface 0.5 to 1.0 cm and moving from base to apex and laterally to the midline, a minimum of three times per lobe. Transfer the contents of the urine collection container into a 50 mL transport tube filled with 800 μL of 0.5M EDTA. The tubes were stored at 2-8°C for a maximum of three days after collection and shipped overnight with standard ice packs.
[0009] Upon receipt, the transport tubes were imme...
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