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A Molecular Marker and Application of a Rapeseed Recessive Genic Sterile Gene Segment

A technique for recessive sterility and molecular markers, applied in the field of molecular markers for the control of recessive sterility gene segments in Brassica napus

Inactive Publication Date: 2011-12-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many molecular markers have been developed in the process of positioning, and some markers have been verified and can be applied to marker-assisted breeding. However, there are still certain limitations in the efficiency and accuracy of selection due to the lack of intragenic molecular markers.

Method used

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  • A Molecular Marker and Application of a Rapeseed Recessive Genic Sterile Gene Segment
  • A Molecular Marker and Application of a Rapeseed Recessive Genic Sterile Gene Segment
  • A Molecular Marker and Application of a Rapeseed Recessive Genic Sterile Gene Segment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing a nucleotide sequence of a gene segment controlling recessive nuclear sterility of Brassica napus and a molecular marker, the steps of which are:

[0040] The applicant's unit has located BnMs3 and Bnrf (Bnesp) in a genetic interval of less than 0.2cM on the N19 and N7 linkage groups of Brassica napus through the primary and fine mapping of the genes BnMs3 and Bnrf that control the recessive sterility of rapeseed Inside.

[0041] A. The Tapidor BAC library was screened by marker screening combined with Southern hybridization, and the target clones JBnB043L23 and JBnB089D05 containing BnMS3 and Bnrf were obtained respectively.

[0042] B. The nucleotide sequences of the candidate gene segments of BnMs3 and Bnrf (Bnesp) were obtained by local sequencing of the target BAC and the combination of further development markers and comparative sequencing. The sequence information is as follows:

[0043] 1) The nucleotide sequence of the BnMs3 gene segmen...

Embodiment 2

[0056] Similar to 987, 195-14 (please refer to fill in the genetic resources table) (excellent parental material bred by the applicant's research group) isotype is BnMs3Ms3rfrf (obtained by genetic analysis, see references for data, Dong Fafa et al., 2010, The same below), use the homozygous sterile line 9012A (please refer to fill in the genetic resources table) (homozygous sterile line provided by researcher Chen Fengxiang of Anhui Academy of Agricultural Sciences) as the donor material, specifically take 987 as an example: a The application of molecular markers for controlling the recessive genetic sterile sterility gene segment of Brassica napus in the molecular marker-assisted insemination of recessive genetic male sterile lines and temporary protection lines of Brassica napus, the steps are:

[0057] In the spring of 2009, the applicant used 9012A as the donor parent, and the excellent hybrid parent 987 bred by the applicant as the recipient parent, and crossed at the flo...

Embodiment 3

[0063] Materials similar to Zhongshuang 9 (please refer to fill in the genetic resource form) (an excellent conventional variety bred by the Chinese Academy of Sciences Oil Plant Selection) with genotype BnMs3Ms3RfRf, using temporary insurance line T45 (please refer to fill in the genetic resource form) (the subject of the applicant The excellent double-low recessive genic male sterile line bred by the group) was used as the donor material, and the markers used for prospect selection are shown in Table 1. A molecular marker controlling the recessive genic male sterility gene segment of Brassica napus was used in the The application of the molecular marker-assisted transfer of the recessive genic male sterile line and the temporary insurance line of rapeseed type, the steps are: the specific steps are as follows:

[0064] 1) In the spring of 2008, the applicant used T45 as the donor parent, and the conventional species Zhongshuang 9 (ZS9) which was widely promoted in production ...

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Abstract

The invention discloses a molecular marker and application of a rapeseed recessive nuclear sterile gene segment. The steps are as follows: A. Positioning BnMs3 and Bnrf in the Brassica oleracea by controlling the location of the rapeseed recessive nuclear sterile gene BnMs3 and Bnrf A genetic interval of less than 0.2cM on the N19 and N7 linkage groups of rapeseed; B. The TapidorBAC library was screened by marker screening combined with Southern hybridization, and the target clones JBnB043L23 and JBnB089D05 containing BnMS3 and Bnrf were obtained; C. BnMs3 was obtained by the sequencing of the target BAC The nucleotide sequence of the candidate gene segment with Bnrf; D, the difference between the nucleotide sequence of the BnMs3 and the allelic recessive gene Bnms3 gene segment according to C step and the difference between BnRf and the allelic recessive gene Bnrf gene segment Differences between nucleotide sequences, development and design of primers, and development of molecular markers for the BnMs3 and BnRf sterile gene segments. The application of molecular markers in the molecular marker-assisted breeding of the recessive genetic male sterile line and the temporary protection line of Brassica napus makes the molecular marker-assisted transfer of the recessive genetic male sterile line and the temporary protection line of rapeseed faster, more accurate and more accurate. efficient.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding. It specifically relates to a molecular marker for controlling recessive nuclear sterility genes (BnMs3 / ms3 and BnRf / rf) in Brassica napus, and also relates to a molecular marker for controlling recessive nuclear sterility genes in Brassica napus The application in the breeding of rapeseed recessive male sterile line and temporary insurance line. Background technique [0002] The utilization of heterosis is considered to be an effective way to increase crop yield and quality, and a good pollination control system is the key to the utilization of heterosis. At present, the ways of utilizing heterosis mainly include artificial emasculation, chemical detasseling, self-incompatibility, and male sterility, among which male sterility is the most widely used. The genetic phenomenon that plants cannot produce normal pollen during sexual reproduction is called male sterility, and male sterility...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 杨光圣洪登峰董发明李季
Owner HUAZHONG AGRI UNIV
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