Method for cultivating triploid dendrobium officinale

Abstract

The invention relates to a method for cultivating triploid dendrobium candidum. The method comprises the following steps of: (1) firstly widely collecting dendrobium candidum seed resources in which good dendrobium candbium is selected; (2) carrying out artificial mutagenesis on the selected dendrobium candbium to obtain autotetraploid dendrobium candbium; (3) carrying out sexual hybridization based on the autotetraploid dendrobium candbium as a female parent and diploid dendrobium candbium as a male parent to obtain triploid dendrobium candbium seeds; (4) carrying out non symbiosis germination culture on the triploid dendrobium candbium seeds; (5) carrying out induction and differentiation culture on the protocorms (PLBs) of the triploid dendrobium candbium seeds; (6) carrying out differentiation on triploid dendrobium candbium mature seedling; (7) transplanting triploid dendrobium candbium seedlings in bottles; and (8) carrying out cultivation management on the triploid dendrobium candbium mature seedling. In the invention, a triploid having the advantages of a polyploid and a hybrid cultivar can be obtained through genome triplication, and the triploid is giant and non substitutable; the yield of dendrobium candbium medicinal material can be greatly improved; and meanwhile, the triploid dendrobium candbium is a permanent hybrid, and hybrid vigor can be fixed.

Description

Technical field: [0001] The invention relates to a method for cultivating triploid Dendrobium officinale. Background technique: [0002] Dendrobium candidum Wall.ex Lindl, also known as black knotweed, is an epiphytic and perennial herbaceous orchid. Mainly distributed in my country's Yunnan, Guizhou, Guangxi, Taiwan and other places. The annual average temperature is 12-18 degrees, the relative humidity is 60-75%, the forest light transmittance is about 60%, and the annual rainfall is 1100-1500mm. It grows in evergreen broad-leaved forests and is epiphytic on tree trunks or limestone. Wild Dendrobium officinale seeds have a very low survival rate and slow growth. It takes 5-7 years to harvest from seed germination to commercialization, resulting in very little yield. In recent years, the natural resources of Dendrobium officinale have been depleted day by day due to over-excavation due to the shortage of market and the deteriorating environment. Dendrobium candidum, commonly...

AI Technical Summary

Problems solved by technology

The survival rate of wild Dendrobium officinale seeds is extremely low, and the growth is slow. It takes 5-7 years to harvest from seed germination to the commercial stage, resulting in very little yield

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