Method for identifying resistance against heterodera glycines

Abstract

The invention relates to a method for identifying soybean cyst nematode resistance. The method is to first cut the tender parts of the soybean vegetative tissues of the susceptible control variety and the variety to be identified to induce rooting culture, and after transplanting and surviving, the seedlings are inoculated with 400 / mL cysts containing eggs and / or second-instar larvae Continue culturing the nematode suspension at 20-30°C. When white cysts appear in the roots of the seedlings, count the number of cysts in the roots of the susceptible control variety and the variety to be identified, and define the disease resistance of the material according to the resistance grading standard. The invention has positive and beneficial effects: the rooting of the vegetative body does not change the genotype, therefore, the resistance of the vegetative body can represent the resistance of the parent material; a part of the vegetative body of the material to be identified is used to replace the whole plant for identification, and the resistant material can be identified Completely preserved; for the group of segregated generations (such as F2), 3 to 5 branches can be taken from a single plant for identification, so as to achieve repeated identification of the segregated population.

Description

technical field [0001] The invention relates to the field of identification of crop disease resistance, in particular to a new method for identification of soybean cyst nematode resistance. Background technique [0002] Soybean cyst nematode (SCN) is a major disease of soybean caused by soybean cyst nematode ( Heterodera glycines ) infection is one of the most prevalent and devastating diseases in soybean production in the world. Soybean cyst nematode disease generally causes a loss of 5-10% of soybean yield, and in serious cases, the yield of the plot can be reduced by more than 30%, and even the grains cannot be harvested. Breeding and promoting disease-resistant soybean varieties is the most cost-effective control measure. Breeding for disease resistance against soybean cyst nematodes is difficult because of the heavy workload of resistance identification, and the existing identification methods are destructive to materials, and it is difficult to preserve breeding mat...

AI Technical Summary

Problems solved by technology

Breeding for disease resistance against soybean cyst nematodes is difficult because of the heavy workload of resistance identification, and the existing identification methods are destructive to materials, and it is difficult to preserve breeding materials identified as resistant