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Culture medium preparation device and method

A medium preparation and culture medium technology, which is applied in the medium preparation device and the field of preparation, can solve the problems of affecting accuracy, difficulty in control, loss of medium components, etc., and achieve the effect of high preparation efficiency, high precision and simple operation

Inactive Publication Date: 2011-10-26
HENAN INST OF SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Animal and plant cell and tissue culture and microbial culture all need culture medium, and existing culture medium preparation method generally has the following problems: (1) solid culture medium needs to boil in advance, and culture medium is easy to splash during boiling process, causes The loss of medium components affects the accuracy; (2) when the medium is subpackaged, it is directly poured into the culture container from the preparation container, which is easy to spill out and the filling volume is difficult to control; (3) the boiled solid medium The process is easy to solidify, resulting in uneven solidification of the culture medium; (3) The entire preparation process is cumbersome, difficult to control, and low in efficiency

Method used

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Embodiment 1

[0018] Embodiment 1 culture medium preparation device

[0019] Such as figure 1 As shown, the culture medium preparation device of the present invention comprises a preparation container 2, a stirring device 11, a support 12, a hollow hose 3 with openings at both ends; the preparation container 2 is placed on the stirring device 11, and the middle part of the hollow hose 3 is fixed on On the support 12, one end of the hollow hose 3, namely the inlet end, is inserted into the bottom of the preparation container 2 with the culture medium, and the other end, namely the outlet end, extends to the lower part of the opening of the culture container 4.

[0020] In this embodiment: the preparation container 2 is a beaker; the stirring device 11 and the support 12 are two components of the magnetic stirrer 1; position; the hollow hose 3 is a rubber hose with a length of 1.2m.

[0021] When the vertical distance between the bottom of the preparation container 2 and the operating platf...

Embodiment 2

[0022] Embodiment 2 culture medium preparation method

[0023] Taking the preparation of MS+1.0mg / L6-BA solid medium as an example, the medium preparation method using the preparation device of the preferred embodiment 1 of the present invention comprises the following steps:

[0024] 1) Add all the required macroelements, trace elements, organic elements, hormones, sucrose and deionized water into a 2L preparation container beaker;

[0025] 2) Place the beaker on a magnetic stirrer and stir until the sucrose dissolves, set the volume to 2L, and adjust the pH value to 5.8;

[0026] 3) Add 7g of agar powder and keep stirring;

[0027] 4) Pass the rubber hose through the arc-shaped device on the bracket of the magnetic stirrer, so that the middle part of the hose is fixed on the bracket to prevent the hose from moving and discounting;

[0028] 5) Put the end of the rubber hose as the inlet end into the bottom of the beaker;

[0029] 6) Place 60 triangular flasks with a capaci...

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Abstract

The invention discloses a culture medium preparation device and method. The culture medium preparation device comprises a preparation container, a stirring device, a bracket and a hollow hose provided with two openings at both ends. The culture medium preparation method is characterized in that all culture medium components are added into the preparation container and are continuously stirred by the stirring device without being decocted previously, so that agar powder components which are unsolvable at normal temperature can be uniformly dispersed and then subpackaged into a culture container through the hollow hose. The culture medium preparation device is simple in structure and easy to manufacture, and can be formed by jointing conventional laboratory equipment. The culture medium preparation method has the advantages of simple operation process and high efficiency. The prepared culture medium has high accuracy and can be solidified uniformly. Thus, the invention can be widely used in colleges and universities, research units, factories and enterprises.

Description

technical field [0001] The invention belongs to the technical field of biological cultivation, and in particular relates to a medium preparation device and a preparation method. Background technique [0002] Animal and plant cell and tissue culture and microbial culture all need culture medium, and existing culture medium preparation method generally has the following problems: (1) solid culture medium needs to boil in advance, and culture medium is easy to splash during boiling process, causes The loss of medium components affects the accuracy; (2) when the medium is subpackaged, it is directly poured into the culture container from the preparation container, which is easy to spill, and the subpackage volume is difficult to control; (3) the boiled solid medium The process is easy to solidify, resulting in uneven coagulation of the culture medium; (3) the entire preparation process has cumbersome steps, is difficult to control, and has low efficiency. Contents of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M27/02
Inventor 刘会超贾文庆陈韵杜晓华刘振威齐安国王少平周秀梅
Owner HENAN INST OF SCI & TECH
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