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Rice protein polypeptide and preparation method thereof

A technology of rice protein and protein, applied in the field of rice protein polypeptide

Active Publication Date: 2013-01-02
WUXI JINNONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that there are still some defects in the enzymatic hydrolysis reaction and its effect in the prior art, and it is far from reaching a relatively ideal result. Therefore, people still need to study and improve this technology.

Method used

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  • Rice protein polypeptide and preparation method thereof
  • Rice protein polypeptide and preparation method thereof
  • Rice protein polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1: The compound enzyme method of the present invention prepares rice protein polypeptide method

[0094] The steps of this method are as follows:

[0095] (1) Mixing

[0096] The rice dregs sold by Yunnan Pu'er Yongji Biotechnology Co., Ltd. were screened in advance with a 150-mesh sieve to obtain rice dregs with a particle size of 150 mesh. Then weigh 2kg protein weight percentage 90% and fat weight percentage 3% rice dregs, according to rice dregs and water weight ratio 1: 7, rice dregs and 14kg water are stirred and mixed to obtain a kind of feed liquid, and then Heating was carried out at a temperature of 70°C.

[0097] (2) Degreasing

[0098] with saturated Ca(OH) 2 Aqueous solution Adjust the pH value of the feed liquid obtained in step (1) to 9.5, stir evenly, and then soak and degrease at a temperature of 75° C. for 30 minutes.

[0099] (3) centrifugal

[0100] Using the centrifuge produced by Xiangtan Centrifuge Co., Ltd., the feed liquid obtai...

Embodiment 2

[0118] Embodiment 2: the method for preparing rice protein polypeptide by compound enzyme method of the present invention

[0119] (1) Mixing

[0120] The rice dregs sold by Yunnan Pu'er Yongji Biotechnology Co., Ltd. were screened in advance with a 100-mesh sieve to obtain rice dregs with a particle size of 100 mesh. Then weigh 2kg protein weight percentage 80% and fat weight percentage 4% rice dregs, according to rice dregs and water weight ratio 2: 9, rice dregs and 9kg water are stirred and mixed to obtain a kind of feed liquid, and then Heating was carried out at a temperature of 75°C.

[0121] (2) Degreasing

[0122] with saturated Ca(OH) 2 Aqueous solution Adjust the pH value of the feed solution obtained in step (1) to 9.7, stir evenly, and soak and degrease at a temperature of 73° C. for 20 minutes.

[0123] (3) centrifugal

[0124] Using the centrifuge produced by Xiangtan Centrifuge Co., Ltd., the feed liquid obtained in step (2) was centrifuged at a speed of 3...

Embodiment 3

[0135] Embodiment 3: the method for preparing rice protein polypeptide by compound enzyme method of the present invention

[0136] The steps of this method are as follows:

[0137] (1) Mixing

[0138] Use a 200-mesh sieve to screen the rice dregs sold by Yunnan Pu'er Yongji Biotechnology Co., Ltd. in advance to obtain rice dregs with a particle size of 200 mesh. Then weigh 2kg protein weight percentage 70% and fat weight percentage 2% rice dregs, according to rice dregs and water weight ratio 3: 8, rice dregs and 5.3kg water are stirred and mixed to obtain a kind of feed liquid, and then Heating was carried out at a temperature of 73°C.

[0139] (2) Degreasing

[0140] with saturated Ca(OH) 2 Aqueous solution Adjust the pH value of the feed solution obtained in step (1) to 10.0, stir evenly, and soak and degrease at a temperature of 70° C. for 40 minutes.

[0141] (3) centrifugal

[0142] Using the centrifuge produced by Xiangtan Centrifuge Co., Ltd., the feed liquid obt...

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Abstract

The invention relates to a rice protein polypeptide and also relates to a composite enzyme preparation method of the rice protein polypeptide. The preparation method comprises the following steps: size mixing, degreasing, centrifuging, homogenizing, enzymolysis reaction, sterilization and enzyme inactivation, centrifugal separation, concentration, drying and the like. The rice protein polypeptideprepared by using the preparation method has a protein content of 80% in percentage by weight and a fat content of 1% or below in percentage by weight, and has high bioactivity; the peptides of whichthe molecular weight distribution is 100-1000 Da account for more than 90% of the total protein content; and the product are completely soluble in water.

Description

【Technical field】 [0001] The invention belongs to the field of rice protein polypeptide preparation. More specifically, the present invention relates to a rice protein polypeptide and a method for preparing the rice protein polypeptide using a complex enzyme method. 【Background technique】 [0002] The content of rice protein in rice is about 7%, which is mainly composed of albumin, globulin, prolamin and glutenin. Rice protein is a high-quality vegetable protein with high nutritional value. Its amino acid composition ratio is reasonable, which is similar to the optimal ratio mode of protein amino acid recommended by FAO / WHO. In addition, the biological value (BV) and protein utility ratio (PER) of rice protein are almost the best among plant proteins. In addition, rice protein is rich in various amino acids, especially the content of lysine ranks first in cereal foods. Therefore, rice protein is an important protein source in people's diet, and its nutritional value is m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K4/10C12P21/06
Inventor 于秋生
Owner WUXI JINNONG BIOTECH CO LTD
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