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Trans-lysine fermentation preparation

A technology of lysine and fermentation method, applied in the directions of fermentation, botanical equipment and methods, microorganism-based methods, etc., can solve the problems such as no reports of enzyme variants, and achieve no safety hazard, improved fermentation yield, and simple steps. Effect

Active Publication Date: 2012-08-15
NINGXIA EPPEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, studies of this enzyme variant have not been reported

Method used

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  • Trans-lysine fermentation preparation
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  • Trans-lysine fermentation preparation

Examples

Experimental program
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Effect test

Embodiment 2

[0041] The activity assay of embodiment 2 escherichia coli and the fermentation experiment of coryneform bacterium

[0042] According to the existing assay method for transhydrogenase activity (see Clarke DM et al. Cloning and expression of the transhydrogenase gene of Escherichia coli.J.Bacteriol., 162:367-373), E.coli transformed with pMS2-cispnt plasmid -tanspnt bacterial strain and the negative Top10F' bacterial strain as a control measure the transhydrogenase activity respectively, and the specific enzyme activity of the found E.coli-tanspnt bacterial strain is 138% higher than that of the negative Top10F' bacterial strain, much higher than the prior art Enzyme specific activity increase rate with wild-type pyridine nucleotide transhydrogenase expressed in .

[0043] The pMS2-tanspnt plasmid is transferred to L-lysine-fermenting coryneform bacteria engineering bacteria (available from the American Type Microorganism Collection (ATCC), product number ATCC 31269) by electro...

Embodiment 3

[0045] The preparation of embodiment 3L-lysine feed additive

[0046] The fermentation broth prepared in Example 2 was separated by a Suntar-III ultrafiltration membrane (available from Suntar Membrane Technology Co., Ltd.). Then, spray the filtrate directly into the fluidized bed dryer in the form of mist and keep the tail gas temperature at 80±3°C, and collect the discharged particles. Sieve the discharged particles, send the particles with a particle size greater than or equal to 1.5mm to the crusher for crushing, and then mix the crushed particles with the particles with a particle size smaller than 1.5mm obtained by screening and spray them into the fluidized bed again Keep the tail gas temperature in the dryer at 80±3°C, collect the discharged particles and sieve out the particles with a particle size of less than 1.5mm, which are L-lysine feed additives. After the particles with a particle size greater than 1.5mm are crushed again, they are mixed with the discharged pa...

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Abstract

The invention provides an L-lysine fermentation method. The method comprises the following steps: introducing polynucleotides which code pyridine nucleotide transhydrogenase in a non-natural sequence into bacteria which can generate L-lysine to make the obtained bacteria to express the pyridine nucleotide transhydrogenase; and cultivating the obtained bacteria under a fermentation condition. The invention also provides intermediate products used in the fermentation method, and further product production with the fermentation, and the like.

Description

technical field [0001] The present invention belongs to the field of amino acid fermentation, in particular, the present invention relates to a fermentation method of L-lysine, which comprises introducing a polynucleotide encoding pyridine nucleotide transhydrogenase in a non-natural sequence into an L-lysine-producing bacteria, so that the obtained bacteria express the pyridine nucleotide transhydrogenase; and culturing the obtained bacteria under fermentation conditions. In addition, the present invention also provides intermediate products used in the fermentation method, and further products produced by the fermentation method. Background technique [0002] L-lysine is an important amino acid raw material, which can be used as condiment, food, feed additive, and also as an effective or auxiliary ingredient in health care products and medicines. It is widely used in food industry, feed industry, pharmaceutical industry and other chemical industries. in industry. At pres...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12P13/08C12N9/10C12N1/21A23K1/16C12R1/15A23K20/142
Inventor 马吉银陈崇安孟刚曹洪程耀东刘鑫
Owner NINGXIA EPPEN BIOTECH
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