Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for screening biological catalyst strain for converting levo-fosfomycin into dextral-fosfomycin

A technology of levofosfomycin and dexfofomycin, which is applied to biochemical equipment and methods, microorganisms, microorganisms, etc., and can solve the problems of screening failures and large numbers of omissions

Inactive Publication Date: 2011-08-17
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a new screening method for transforming D-fosfomycin into Levofosfomycin catalytic strains, to solve the problems in the prior art that the screening work fails or omits a large number of bacterial strains with catalytic ability, and the present invention can Significantly improve the sensitivity and efficiency of screening

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for screening biological catalyst strain for converting levo-fosfomycin into dextral-fosfomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] First, inoculate the beef extract peptone liquid medium with Escherichia coli, cultivate it at 37°C for 24 hours, and prepare the bacterial suspension of Escherichia coli strain, so that the bacterial concentration is 10 8 -10 10 bacteria / ml, which is made into a high-concentration sensitive bacteria suspension.

[0028] Then the catalytic bacterial strain to be screened may be transformed into levofosfomycin (through these two characteristics of levofosfomycin resistance and / or dextrofosfomycin utilization, use conventional techniques to preliminarily judge whether the bacterial strain is Potentially transformable bacterial strains) were inoculated in the catalytic medium, and after 72 hours of shaking flask culture, the fermentation broth was drawn, and the bacteria in the fermentation broth were removed by filtration through a 0.45 μm pore size filter membrane to prepare a sterile fermentation broth of the bacterial strain to be screened.

[0029] Then, mix the abov...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biomedicines and biology, in particular to a high-sensitivity method for screening a strain for catalyzing the conversion of levo-fosfomycin into dextral-fosfomycin, which comprises: firstly, preparing bacterial suspension of a dextral-fosfomycin sensitive strain, wherein the bacterial concentration is 10<8> to 10<10> / ml; secondly, adding the bacterial suspension of the dextral-fosfomycin sensitive strain into sterile fermentation liquid of catalyst strain to be screened, which is filtered by a filter membrane with an aperture of 0.45 mu m to remove bacteria, collecting fermentation liquid at the starting time of culture and after a certain period of culture, and measuring the absorbance of the collected fermentation liquid by using a 600-nanometer spectrophotometer; and selecting fermentation liquor of which the absorbance increases the least or less after a period of culture, and further verifying if the original strain to be screened has the capability of converting levo-fosfomycin into dextral-fosfomycin. When the method is used, the sensitivity of the method for screening the biological catalyst strain for converting levo-fosfomycin into dextral-fosfomycin is improved greatly, and the screening efficiency is improved greatly for the detection method is simple and easy to implement.

Description

technical field [0001] The invention relates to the fields of biomedicine and biotechnology, in particular to a highly sensitive screening method for catalyzing D-fosfomycin to L-fosfomycin bacterial strains. Background technique [0002] Levofosfomycin [(-)-cis-(1R,2S)-1,2-epoxypropyl phosphate, here abbreviated as FOM] is a broad-spectrum antibiotic. It has a unique chemical structure and antibacterial mechanism, not only has no cross-resistance with other antibiotics, but also mostly has a synergistic effect. At the same time, because fosfomycin also has the advantages of stable physical and chemical properties, safety, low toxicity, and no antigenicity, it has been widely used in clinical treatment and is called a new antibiotic in the 21st century by the international medical and pharmaceutical circles. [0003] At present, what levofosfomycin production technique adopts is chemical synthesis method, and this technique yield is lower (less than 20%), and the main reaso...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/00G01N21/31
Inventor 徐慧杨伟超王若超周丽沙韩斯琴孔双张大鹏纪悦周凯赵华季光辉金玉坤
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com