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Method and processe for orientating, sampling and collecting seed tissues from individual seed

A seed and sample technology, applied in the direction of sampling devices, preparation of test samples, botanical equipment and methods, etc., can solve problems that have not been satisfactorily processed or balanced, and achieve the effect of reducing materials

Active Publication Date: 2011-06-15
PIONEER HI BRED INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-throughput methods may require relatively fast operations, but with relatively high precision and low risk of contamination, so that they must be made slower than technic

Method used

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  • Method and processe for orientating, sampling and collecting seed tissues from individual seed
  • Method and processe for orientating, sampling and collecting seed tissues from individual seed
  • Method and processe for orientating, sampling and collecting seed tissues from individual seed

Examples

Experimental program
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Effect test

Embodiment approach 1

[0098] a) Appliances ( Figure 1A and 1C )

[0099] Figure 1A Illustrated is a system 10 that separates seeds that have been dehulled from an ear of corn, orients each seed about a predetermined direction, successively conveys each seed under a laser beam to cut a sample section from the seed, and The seeds and their corresponding sample clips are sent into indexed vessels.

[0100] Such as Figure 1B As shown, the embodiment of system 10 is implemented by pretreatment of corn kernels. An iron-based coating 2 is applied (for example by brush or spray) to the exterior of the ear of corn 1 . The ear is subsequently husked to release the single seed 3 . Because the seed is coated on the ear 1, the iron-based coating 2 essentially only covers the outer or crown portion of the single seed 3.

[0101] Place the dehulled, coated seeds in a seed separator (in Figure 1D An example is shown separately). Seed separators are well known in the art and are commercially available fr...

Embodiment approach 2

[0171] 2. Implementation scheme 2 ( Figure 1C )

[0172] As will be appreciated, the basic idea of ​​magnetic positioning, orientation and laser cutting can be achieved in a variety of different ways and with a variety of different components and methods. Figure 1C shows up Figure 1A Alternative embodiments of embodiments. A similar buffer wheel 14 is shown having a separate receiver 18 turned by a motor 17 with a shaft 16 . In addition, a fixed disc 21 with a single opening above the chute 22 retains the seeds in the respective wells 18 of the rotating buffer wheel 14 except in the disc 21 which is in line with the chute 22 and which opens directly above the well. In this manner, the individual seeds 3 are dropped one at a time and at intervals.

[0173] exist Figure 1C In , a vertically disposed wheel 72 has magnets 34 at the spaced positions shown. As the wheel 72 is turned by the chute 22 , the separated seeds settle in place coincident with the position of each ...

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Abstract

Apparatus, methods, and systems for high throughput useful sampling of seed, wherein viability is selectively maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed using a hand-held and manually operated tool having one or more cutting edges. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is held in pre-determined orientation to facilitate efficient and accurate sampling.

Description

[0001] This application is a Chinese patent application with an application date of November 13, 2007, an application number of 200780049324.2, and an invention title of "Method, process and automation device for orientation, sampling and collection of seed tissue from a single seed" divisional application. 【Technical field】 [0002] The present invention relates to obtaining tissue samples from single seeds in an efficient manner. 【Background technique】 [0003] In crop breeding or plant advancement experiments, it is common practice to grow plants from seeds of known sources. Seeds are planted in experimental plots, growth chambers, greenhouses, or other growing conditions where they are either cross-pollinated with other plants of known origin, or self-pollinated. The resulting seeds are the offspring of the two parent plants or the self-pollinated plants and are harvested, processed and planted to continue the crop breeding cycle. To aid in the cultivation or to facili...

Claims

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Application Information

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IPC IPC(8): G01N1/28B26B13/00A01G3/00G01N1/04G01N35/00A01C1/02B26B17/00A24F13/24
CPCG01N1/28G01N1/04A01C1/025A01G3/00B26B17/00A24F13/24G01N35/00G01N2033/0077G01N35/0098B26B13/00G01N2035/00564
Inventor J·科普D·库尔斯
Owner PIONEER HI BRED INT INC
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