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High-fat Chlorella culture solution for promoting rapid growth and preparation method thereof

A technology for promoting growth and chlorella, applied in the biological field, can solve the problems of limiting chlorella photosynthesis and low chlorella biomass

Inactive Publication Date: 2011-06-15
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technology of autotrophic culture of Chlorella has been mature, but there is a problem that light is blocked from entering the culture after the Chlorella cells are increased to a certain concentration, thus limiting the photosynthesis of Chlorella, resulting in the growth of the cultured Chlorella low biomass

Method used

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  • High-fat Chlorella culture solution for promoting rapid growth and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Put 30kg of bamboo vinegar into a 1000L stirred tank, then add 15kg of sodium nitrate, 20kg of potassium nitrate, 15kg of potassium dihydrogen phosphate and 15kg of sodium chloride, then add 1kg of ferric chloride, 1kg of boric acid, 1kg of copper sulfate, 1kg of manganese sulfate, 0.5kg of zinc sulfate and 0.5kg of ammonium molybdate, stir evenly, then add 900kg of water, stir until all substances are dissolved, and the final product is obtained.

Embodiment 2

[0024] Put 50kg of bamboo vinegar into a 1000L stirred tank, then add 25kg of potassium nitrate, 25kg of magnesium sulfate, 25kg of calcium chloride and 25kg of sodium chloride, then add 10kg of ferric chloride, 5kg of boric acid and 5kg of copper sulfate, stir evenly, and add 10kg of Add 820kg of water to the soil extract and stir until all substances are dissolved to form the final product.

Embodiment 3

[0026] Put 38kg of bamboo vinegar into the 1000L stirred tank, then add 17kg of sodium nitrate, 17kg of dipotassium hydrogen phosphate, 17kg of calcium chloride, 17kg of potassium dihydrogen phosphate and 17kg of sodium chloride, then add 2kg of copper sulfate, 2kg of manganese sulfate, 2kg of Zinc sulfate and 2kg ammonium molybdate, stir evenly, add 2kg soil extract, then add 867kg water, stir until all substances are dissolved, and the final product is served.

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Abstract

The invention relates to a high-fat Chlorella culture solution for promoting rapid growth and a preparation method thereof, belonging to the field of a biotechnology. The culture solution comprises the following components in percent by weight: 1-8% of bamboo vinegar, 4-15% of macronutrient, 0.2-0.5% of micronutrient, 0-1.5% of soil extract and 78-94% of water, wherein the macronutrient is one ofsodium nitrate, dipotassium hydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, potassium dihydrogen phosphate and sodium chloride or a mixture thereof, and the micronutrient is one of ferric chloride, boric acid, copper sulfate, manganese sulfate, zinc sulfate and ammonium molybdate or mixture thereof. Since the bamboo vinegar serving as a synergic ingredient is added into the culture solution, the content of the fat generated during the process of the carbon dioxide immobilization using algae is increased. The high-fat Chlorella culture solution is simple in preparation method, low in production cost, and convenient in operation, and can be used for greatly increasing the content of the fat in Chlorella; and under the same conditions, the purity of the cultured algae is very high, and the culture cost for large-scale exploitation and utilization of the Chlorella is greatly reduced.

Description

[0001] technical field [0002] The invention belongs to the field of biotechnology, and in particular relates to a high-fat chlorella culture solution for rapid growth promotion and a preparation method thereof. Background technique [0003] [0004] Chlorella is a general unicellular green algae of the Chlorella phylum Chlorella, which is a spherical single-cell freshwater algae with a diameter of 3 to 8 microns. It is one of the earliest life on earth and appeared in more than 2 billion Years ago, the genes remained unchanged. It is a highly efficient photosynthetic plant that grows and reproduces with photosynthetic autotrophy and is widely distributed. It is one of the lowest and oldest plants, it reproduces quickly, has a high oil content, and is an important source of biofuels. Chlorella is rich in protein, lipid, polysaccharide, dietary fiber, vitamins, trace elements and active metabolites, which can prevent and treat peptic ulcer, anti-tumor, enhance immunity, an...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 黄有军张启香闫道良胡君艳黄坚钦郑炳松夏国华陈国娟童森淼
Owner ZHEJIANG FORESTRY UNIVERSITY
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